SPARCL1 suppresses metastasis in prostate cancer. Issue 6 (20th July 2013)

• Record Type: Journal Article
• Authors: Xiang, Yuzhu; Qiu, Qingchao; Jiang, Ming; Jin, Renjie; Lehmann, Brian D.; Strand, Douglas W.; Jovanovic, Bojana; DeGraff, David J.; Zheng, Yi; Yousif, Dina A.; Simmons, Christine Q.; Case, Thomas C.; Yi, Jia; Cates, Justin M.; Virostko, John; He, Xiusheng; Jin, Xunbo; Hayward, Simon W.; Matusik, Robert J.; George, Alfred L.; Yi, Yajun
• Is Part Of: Molecular oncology. Volume 7:Issue 6(2013:Dec.)
• Page Start: 1019
• Page End: 1030
• Publication Date: 2013-07-20
• Subjects: Prostate cancer -- Gene expression signature -- Meta-analysis -- Metastasis -- SPARCL1 function in vivo; Cancer -- Molecular aspects -- Periodicals; 616.994005
• DOI: 10.1016/j.molonc.2013.07.008
• Languages: English
• ISSNs: 1574-7891

Abstract

Abstract : Purpose: Metastasis, the main cause of death from cancer, remains poorly understood at the molecular level. Experimental design: Based on a pattern of reduced expression in human prostate cancer tissues and tumor cell lines, a candidate suppressor gene (SPARCL1) was identified. We used in vitro approaches to determine whether overexpression of SPARCL1 affects cell growth, migration, and invasiveness. We then employed xenograft mouse models to analyze the impact of SPARCL1 on prostate cancer cell growth and metastasis in vivo. Results: SPARCL1 expression did not inhibit tumor cell proliferation in vitro. By contrast, SPARCL1 did suppress tumor cell migration and invasiveness in vitro and tumor metastatic growth in vivo, conferring improved survival in xenograft mouse models. Conclusions: We present the first in vivo data suggesting that SPARCL1 suppresses metastasis of prostate cancer. Highlights: We identify a candidate suppressor gene (SPARCL1) from a cancer prognostic signature. We examine expression patterns of SPARCL1 in human prostate tissues and cell lines. Overexpression of SPARCL1 does not affect tumor cell growth in vitro. SPARCL1 suppresses tumor cell migration, invasiveness, and metastasis.