Enzyme Shielding in an Enzyme‐thin and Soft Organosilica Layer. (9th April 2016)

• Record Type: Journal Article
• Authors: Correro, M. Rita; Moridi, Negar; Schützinger, Hansjörg; Sykora, Sabine; Ammann, Erik M.; Peters, E. Henrik; Dudal, Yves; Corvini, Philippe F.‐X.; Shahgaldian, Patrick
• Is Part Of: Angewandte Chemie. Volume 128:Number 21(2016)
• Page Start: 6393
• Page End: 6397
• Publication Date: 2016-04-09
• Subjects: Enzymkatalyse -- Nanobiokatalysatoren -- Nanopartikel -- Organosilciumoxid -- Selbstorganisation; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201600590
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: The fragile nature of most enzymes is a major hindrance to their use in industrial processes. Herein, we describe a synthetic chemical strategy to produce hybrid organic/inorganic nanobiocatalysts; it exploits the self‐assembly of silane building blocks at the surface of enzymes to grow an organosilica layer, of controlled thickness, that fully shields the enzyme. Remarkably, the enzyme triggers a rearrangement of this organosilica layer into a significantly soft structure. We demonstrate that this change in stiffness correlates with the biocatalytic turnover rate, and that the organosilica layer shields the enzyme in a soft environment with a markedly enhanced resistance to denaturing stresses.