Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short‐term exposure to tobacco smoke. Issue 1 (23rd August 2014)

• Record Type: Journal Article
• Authors: Niimori-Kita, Kanako; Ogino, Kiyoshi; Mikami, Sayaka; Kudoh, Shinji; Koizumi, Daikai; Kudoh, Noritaka; Nakamura, Fumiko; Misumi, Masahiro; Shimomura, Tadasuke; Hasegawa, Koki; Usui, Fumihiko; Nagahara, Noriyuki; Ito, Takaaki
• Is Part Of: FEBS open bio. Volume 4:Issue 1(2014)
• Page Start: 746
• Page End: 754
• Publication Date: 2014-08-23
• Subjects: Tobacco smoke exposure -- Nuclear phosphoprotein -- Phosphoproteomic analysis -- Signaling pathways; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.fob.2014.08.002
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Smoking is a risk factor for lung diseases, including chronic obstructive pulmonary disease and lung cancer. However, the molecular mechanisms mediating the progression of these diseases remain unclear. Therefore, we sought to identify signaling pathways activated by tobacco‐smoke exposure, by analyzing nuclear phosphoprotein expression using phosphoproteomic analysis of lung tissue from mice exposed to tobacco smoke. Sixteen mice were exposed to tobacco smoke for 1 or 7 days, and the expression of phosphorylated peptides was analyzed by mass spectrometry. A total of 253 phosphoproteins were identified, including FACT complex subunit SPT16 in the 1‐day exposure group, keratin type 1 cytoskeletal 18 (K18), and adipocyte fatty acid‐binding protein, in the 7‐day exposure group, and peroxiredoxin‐1 (OSF3) and spectrin β chain brain 1 (SPTBN1), in both groups. Semi‐quantitative analysis of the identified phosphoproteins revealed that 33 proteins were significantly differentially expressed between the control and exposed groups. The identified phosphoproteins were classified according to their biological functions. We found that the identified proteins were related to inflammation, regeneration, repair, proliferation, differentiation, morphogenesis, and response to stress and nicotine. In conclusion, we identified proteins, including OSF3 and SPTBN1, as candidate tobacco smoke‐exposure markers; our results provide insights into the mechanisms of tobacco smoke‐induced diseases. Abstract : We analyzed nuclear phosphoprotein expression activated by tobacco smoke exposure. 253 phosphoproteins were identified in 1‐day and 7‐day exposure groups. Of these, 33 were significantly differentially expressed in control and exposed groups. Identified proteins were related to inflammation, response to stress and nicotine. OSF3 and spectrin β chain were identified as candidate tobacco smoke markers.