Chronic exposure of a freshwater mussel to elevated pCO2: Effects on the control of biomineralization and ion‐regulatory responses. (13th December 2017)

• Record Type: Journal Article
• Authors: Jeffrey, Jennifer D.; Hannan, Kelly D.; Hasler, Caleb T.; Suski, Cory D.
• Is Part Of: Environmental toxicology and chemistry. Volume 37:Number 2(2018)
• Page Start: 538
• Page End: 550
• Publication Date: 2017-12-13
• Subjects: Chitin synthase -- Na+‐K+‐ATPase -- Calmodulin -- Mollusk toxicology -- Freshwater toxicology -- Benthic macroinvertebrates; Pollution -- Environmental aspects -- Periodicals; Environmental chemistry -- Periodicals; 615.902
• DOI: 10.1002/etc.3991
• Languages: English
• ISSNs: 0730-7268

Abstract

Abstract: Freshwater mussels may be exposed to elevations in mean partial pressure of carbon dioxide ( p CO2 ) caused by both natural and anthropogenic factors. The goal of the present study was to assess the effects of a 28‐d elevation in p CO2 at 15 000 and 50 000 μatm on processes associated with biomineralization, ion regulation, and cellular stress in adult Lampsilis siliquoidea (Barnes, 1823). In addition, the capacity for mussels to compensate for acid‐base disturbances experienced after exposure to elevated p CO2 was assessed over a 14‐d recovery period. Overall, exposure to 50 000 μatm p CO2 had more pronounced physiological consequences compared with 15 000 μatm p CO2 . Over the first 7 d of exposure to 50 000 μatm p CO2, the mRNA abundance of chitin synthase ( cs ), calmodulin ( cam ), and calmodulin‐like protein ( calp ) were significantly affected, suggesting that shell formation and integrity may be altered during p CO2 exposure. After the removal of the p CO2 treatment, mussels may compensate for the acid‐base and ion disturbances experienced during p CO2 exposure, and transcript levels of some regulators of biomineralization (carbonic anhydrase [ ca ], cs, cam, calp ) as well as ion regulation (na + ‐k + ‐ATPase [ nka ]) were modulated. Effects of elevated p CO2 on heat shock protein 70 ( hsp70 ) were limited in the present study. Overall, adult L. siliquoidea appeared to regulate factors associated with the control of biomineralization and ion regulation during and/or after the removal of p CO2 exposure. Environ Toxicol Chem 2018;37:538–550. © 2017 SETAC