Long range epigenetic silencing is a trans‐species mechanism that results in cancer specific deregulation by overriding the chromatin domains of normal cells. Issue 6 (30th August 2013)

• Record Type: Journal Article
• Authors: Forn, Marta; Muñoz, Mar; Tauriello, Daniele V.F.; Merlos-Suárez, Anna; Rodilla, Verónica; Bigas, Anna; Batlle, Eduard; Jordà, Mireia; Peinado, Miguel A.
• Is Part Of: Molecular oncology. Volume 7:Issue 6(2013:Dec.)
• Page Start: 1129
• Page End: 1141
• Publication Date: 2013-08-30
• Subjects: Gene silencing -- DNA methylation -- Colorectal cancer -- Chromatin marks -- Histone modification -- Long range epigenetic silencing -- Cell differentiation; Cancer -- Molecular aspects -- Periodicals; 616.994005
• DOI: 10.1016/j.molonc.2013.08.008
• Languages: English
• ISSNs: 1574-7891

Abstract

Abstract : DNA methylation and chromatin remodeling are frequently implicated in the silencing of genes involved in carcinogenesis. Long Range Epigenetic Silencing (LRES) is a mechanism of gene inactivation that affects multiple contiguous CpG islands and has been described in different human cancer types. However, it is unknown whether there is a coordinated regulation of the genes embedded in these regions in normal cells and in early stages of tumor progression. To better characterize the molecular events associated with the regulation and remodeling of these regions we analyzed two regions undergoing LRES in human colon cancer in the mouse model. We demonstrate that LRES also occurs in murine cancer in vivo and mimics the molecular features of the human phenomenon, namely, downregulation of gene expression, acquisition of inactive histone marks, and DNA hypermethylation of specific CpG islands. The genes embedded in these regions showed a dynamic and autonomous regulation during mouse intestinal cell differentiation, indicating that, in the framework considered here, the coordinated regulation in LRES is restricted to cancer. Unexpectedly, benign adenomas in ApcMin/+ mice showed overexpression of most of the genes affected by LRES in cancer, which suggests that the repressive remodeling of the region is a late event. Chromatin immunoprecipitation analysis of the transcriptional insulator CTCF in mouse colon cancer cells revealed disrupted chromatin domain boundaries as compared with normal cells. Malignant regression of cancer cells by in vitro differentiation resulted in partial reversion of LRES and gain of CTCF binding. We conclude that genes in LRES regions are plastically regulated in cell differentiation and hyperproliferation, but are constrained to a coordinated repression by abolishing boundaries and the autonomous regulation of chromatin domains in cancer cells. Highlights: Long range epigenetic silencing (LRES) is a mechanism of gene inactivation in cancer. Human and murine intestinal cancers show LRES with similar epigenetic features. Early adenomas show upregulation of genes that will be silenced in advanced stages. LRES overrides autonomous gene regulation by loss of chromatin architecture. Malignant regression results in partial reversion of LRES.