Computer aided prognosis for cell death categorization and prediction in vivo using quantitative ultrasound and machine learning techniques. Issue 12 (10th November 2016)

• Record Type: Journal Article
• Authors: Gangeh, M. J.; Hashim, A.; Giles, A.; Sannachi, L.; Czarnota, G. J.
• Is Part Of: Medical physics. Volume 43:Issue 12(2016)
• Page Start: 6439
• Page End: 6454
• Publication Date: 2016-11-10
• Subjects: biomedical ultrasonics -- cancer -- cellular biophysics -- feature extraction -- image texture -- learning (artificial intelligence) -- medical image processing -- radiation therapy -- tumours -- ultrasonic therapy; Ultrasonography -- Cell processes -- Cancer -- Therapeutic applications; Diagnosis using ultrasonic, sonic or infrasonic waves -- Radiation therapy -- Ultrasound therapy -- In which a programme is changed according to experience gained by the computer itself during a complete run; Learning machines -- Digital computing or data processing equipment or methods, specially adapted for specific applications -- Image data processing or generation, in general -- Analysis of texture -- Inference methods or devices; cancer therapy -- computer aided prognosis -- kernel methods -- microbubbles -- quantitative ultrasound; Cancer -- Ultrasonography -- Fluid bubbles -- Radiation treatment -- Dosimetry -- Backscattering -- Ultrasonic effects -- Radiofrequency spectra; Medical physics -- Periodicals; Medical physics; Geneeskunde; Natuurkunde; Toepassingen; Biophysics; Periodicals; Periodicals; Electronic journals; 610.153
• DOI: 10.1118/1.4967265
• Languages: English
• ISSNs: 0094-2405

Abstract

Abstract : Purpose: At present, a one‐size‐fits‐all approach is typically used for cancer therapy in patients. This is mainly because there is no current imaging‐based clinical standard for the early assessment and monitoring of cancer treatment response. Here, the authors have developed, for the first time, a complete computer‐aided‐prognosis (CAP) system based on multiparametric quantitative ultrasound (QUS) spectroscopy methods in association with texture descriptors and advanced machine learning techniques. This system was used to noninvasively categorize and predict cell death levels in fibrosarcoma mouse tumors treated using ultrasound‐stimulated microbubbles as novel endothelial‐cell radiosensitizers. Methods: Sarcoma xenograft tumor‐bearing mice were treated using ultrasound‐stimulated microbubbles, alone or in combination with x‐ray radiation therapy, as a new antivascular treatment. Therapy effects were assessed at 2–3, 24, and 72 h after treatment using a high‐frequency ultrasound. Two‐dimensional spectral parametric maps were generated using the power spectra of the raw radiofrequency echo signal. Subsequently, the distances between "pretreatment" and "post‐treatment" scans were computed as an indication of treatment efficacy, using a kernel‐based metric on textural features extracted from 2D parametric maps. A supervised learning paradigm was used to either categorize cell death levels as low, medium, or high using a classifier, or to "continuously" predict the levels of cell death using a regressor. Results: The developed CAP system performed at a high level for the classification of cell death levels. The area under curve of the receiver operating characteristic was 0.87 for the classification of cell death levels to both low/medium and medium/high levels. Moreover, the prediction of cell death levels using the proposed CAP system achieved a good correlation ( r = 0.68, p < 0.001) with histological cell death levels as the ground truth. A statistical test of significance between individual treatment groups with the corresponding control group demonstrated that the predicted levels indicated the same significant changes in cell death as those indicated by the ground‐truth levels. Conclusions: The technology developed in this study addresses a gap in the current standard of care by introducing a quality control step that generates potentially actionable metrics needed to enhance treatment decision‐making. The study establishes a noninvasive framework for quantifying levels of cancer treatment response developed preclinically in tumors using QUS imaging in conjunction with machine learning techniques. The framework can potentially facilitate the detection of refractory responses in patients to a certain cancer treatment early on in the course of therapy to enable switching to more efficacious treatments.