Optogenetic Control of Voltage‐Gated Calcium Channels. Issue 24 (16th April 2018)

• Record Type: Journal Article
• Authors: Ma, Guolin; Liu, Jindou; Ke, Yuepeng; Liu, Xin; Li, Minyong; Wang, Fen; Han, Gang; Huang, Yun; Wang, Youjun; Zhou, Yubin
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 24(2018)
• Page Start: 7019
• Page End: 7022
• Publication Date: 2018-04-16
• Subjects: calcium signaling -- ion channels -- optogenetics -- protein engineering -- synthetic biology; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201713080
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Voltage‐gated Ca 2+ (CaV ) channels mediate Ca 2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded CaV channel modulators, to enable photo‐tunable modulation of CaV channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for CaV channels could find broad applications in interrogating a wide range of CaV ‐mediated physiological processes. Abstract : Light relief : A reversibly photoactivatable voltage‐gated calcium (CaV ) channel modulator, termed optoRGK, was developed. optoRGK enables photo‐tunable regulation of CaV channel activity and Ca 2+ entry in excitable mammalian cells, such as cardiomyocytes, and could find applications in interrogating a range of CaV ‐mediated physiological processes.