Oenothein B inhibits human non-small cell lung cancer A549 cell proliferation by ROS-mediated PI3K/Akt/NF-κB signaling pathway. (25th January 2019)

• Record Type: Journal Article
• Authors: Pei, Xiaodong; Xiao, Junsong; Wei, Guijiang; Zhang, Yumeng; Lin, Feng; Xiong, Zhaocheng; Lu, Lan; Wang, Xueli; Pang, Guangfu; Jiang, Yan; Jiang, Lihe
• Is Part Of: Chemico-biological interactions. Volume 298(2019)
• Page Start: 112
• Page End: 120
• Publication Date: 2019-01-25
• Subjects: Oenothein B -- A549 cell -- ROS -- PI3K/Akt -- NF-kB; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2018.09.021
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Oenothein B has a wide range of biological activities. The present study probed into the underlying mechanism on how Oenothein B inhibits the proliferation of a lung cancer line A549. Our results showed that Oenothein B effectively inhibited the proliferation of A549 cells by inducing apoptosis and arresting cells at G1 stage. Furthermore, Oenothein B not only increased the level of intracellular reactive oxygen species (ROS), but also induced the upregulation of intracellular apoptotic triggers (cleavage caspase-3, PARP, cytochrome c level in the cytosol, Bax). Moreover, ROS inhibitor (N-acetyl-L-cystein, NAC) and PI3K agonist (Insulin-like growth factor 1, IGF-1) could resist cell proliferation inhibition induced by Oenothein B, respectively. ROS inhibitor significantly abrogated the activation of caspase 3/7 and 9 in the presence of Oenothein B. Additionally, suppression of p-PI3K and p-Akt, p-NF-κB by Oenothein B could be compensated by treatment with ROS inhibitor. To summarize, these results demonstrated that Oenothein B was able to prevent cell proliferation probably via ROS-mediated PI3K/Akt/NF-κB signaling pathway. Graphical abstract: Highlights: Oenothein B increases the level of ROS and induces some apoptotic proteins expression. ROS inhibitor and PI3K agonist could resist cell proliferation inhibition induced by Oenothein B, respectively. The protein levels of p-PI3K and p-Akt, p-NF-kB suppressed by Oenothein B could be offset by ROS inhibitor. Oenothein B can prevent cell proliferation maybe via ROS-mediated PI3K/Akt/NF-kB signaling pathway.