NFAT1 promotes intratumoral neutrophil infiltration by regulating IL8 expression in breast cancer. Issue 6 (19th February 2015)

• Record Type: Journal Article
• Authors: Kaunisto, Aura; Henry, Whitney S.; Montaser-Kouhsari, Laleh; Jaminet, Shou-Ching; Oh, Eun-Yeong; Zhao, Li; Luo, Hongbo R.; Beck, Andrew H.; Toker, Alex
• Is Part Of: Molecular oncology. Volume 9:Issue 6(2015:Jun.)
• Page Start: 1140
• Page End: 1154
• Publication Date: 2015-02-19
• Subjects: Nuclear factor of activated T cells 1 -- Interleukin -- Breast cancer -- Neutrophil infiltration; Cancer -- Molecular aspects -- Periodicals; 616.994005
• DOI: 10.1016/j.molonc.2015.02.004
• Languages: English
• ISSNs: 1574-7891

Abstract

Abstract : NFAT transcription factors are key regulators of gene expression in immune cells. In addition, NFAT1‐induced genes play diverse roles in mediating the progression of various solid tumors. Here we show that NFAT1 induces the expression of the IL8 gene by binding to its promoter and leading to IL8 secretion. Thapsigargin stimulation of breast cancer cells induces IL8 expression in an NFAT‐dependent manner. Moreover, we show that NFAT1‐mediated IL8 production promotes the migration of primary human neutrophils in vitro and also promotes neutrophil infiltration in tumor xenografts. Furthermore, expression of active NFAT1 effectively suppresses the growth of nascent and established tumors by a non cell‐autonomous mechanism. Evaluation of breast tumor tissue reveals that while the levels of NFAT1 are similar in tumor cells and normal breast epithelium, cells in the tumor stroma express higher levels of NFAT1 compared to normal stroma. Elevated levels of NFAT1 also correlate with increased neutrophil infiltrate in breast tumors. These data point to a mechanism by which NFAT1 orchestrates the communication between breast cancer cells and host neutrophils during breast cancer progression. Highlights: NFAT1 is a transcriptional regulator of IL8 in breast cancer. Active NFAT1 promotes IL8 production and neutrophil migration. Active NFAT1 suppresses tumor growth in an IL8‐independent mechanism. NFAT1 levels correlate with neutrophil infiltration in breast tumor samples.