Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9. Issue 1 (10th May 2013)

• Record Type: Journal Article
• Authors: He, Ping; Kuhara, Hanako; Tachibana, Isao; Jin, Yingji; Takeda, Yoshito; Tetsumoto, Satoshi; Minami, Toshiyuki; Kohmo, Satoshi; Hirata, Haruhiko; Takahashi, Ryo; Inoue, Koji; Nagatomo, Izumi; Kida, Hiroshi; Kijima, Takashi; Naka, Tetsuji; Morii, Eiichi; Kawase, Ichiro; Kumanogoh, Atsushi
• Is Part Of: FEBS open bio. Volume 3:Issue 1(2013)
• Page Start: 225
• Page End: 230
• Publication Date: 2013-05-10
• Subjects: Small cell lung cancer -- Proteomics -- Tetraspanin -- CD9 -- Calretinin -- Apoptosis; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.fob.2013.04.005
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : A majority of small cell lung cancer (SCLC) cells lack a metastasis suppressor, tetraspanin CD9, and CD9 expression promotes their apoptosis. By a proteomics‐based approach, we compared an SCLC cell line with its CD9 transfectant and found that a calcium‐binding neuronal protein, calretinin, is upregulated in CD9‐positive SCLC cells. Ectopic or anticancer drug‐induced CD9 expression upregulated calretinin, whereas CD9 knockdown down‐regulated calretinin in SCLC cells. When calretinin was knocked down, CD9‐positive SCLC cells revealed increased Akt phosphorylation and decreased apoptosis. These results suggest that CD9 positively regulates the expression of calretinin that mediates proapoptotic effect in SCLC cells. Abstract : Most small cell lung cancer (SCLC) cells lack the metastatic suppressor CD9. An SCLC cell line and its CD9 transfectant were compared by a proteomics approach. Calretinin is upregulated in CD9 (+) SCLC cells. Calretinin is a proapoptotic protein in CD9 (+) SCLC cells.