Effect of cDMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation. Issue 3 (18th April 2018)

• Record Type: Journal Article
• Authors: Tuncer Budanur, Damla; Zibandeh, Noushin; Genç, Deniz; Gökalp, Muazzez; Kaşali, Kamber; Akkoç, Tunç; Sepet, Elif
• Is Part Of: Dental traumatology. Volume 34:Issue 3(2018)
• Page Start: 188
• Page End: 200
• Publication Date: 2018-04-18
• Subjects: cell viability -- ectoine -- fibroblastic differentiation -- osteogenic differentiation -- periodontal ligament cells; Teeth -- Wounds and injuries -- Periodicals; Dentistry, Operative -- Periodicals; Traumatology -- Periodicals; Endodontics -- Periodicals; 617.6
• DOI: 10.1111/edt.12396
• Languages: English
• ISSNs: 1600-4469

Abstract

Abstract: Background/Aim: Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators' effects on viability or differentiation of dental stem cells. The aim of this study was to investigate the effect of Ectoine as a storage media on the viability and differentiation potential of human periodontal ligament mesenchymal stem cells (hPDLMSCs). Materials and Methods: hPDLMSCs were obtained from impacted third molar teeth. The cells were isolated, submitted to trilineage differentiation, and characterized by flow cytometer (FC). hPDLMSCs were incubated with different media containing Ectoine, complete DMEM (cDMEM), Ectoine+cDMEM, milk, and tap water for 2, 6, 12, and 24 h. The cells were analyzed by FC for viability, early‐apoptosis, late apoptosis, and necrosis rates. Osteogenic and fibroblastic differentiation in hPDLMSCs were investigated by Alizarin red stain and vimentin expression. Results: All treated groups showed significant decreases in cell viability after 2 h. Significant increases were detected in the number of dead cells between 2 and 12 h in all groups except the Ectoine+cDMEM group. The deposition of mineral matrix nodules was significantly higher in cells cultured with Ectoine+cDMEM compared with the other media. Higher vimentin expressions were detected in cells cultured with cDMEM and Ectoine+cDMEM media, respectively. Conclusions: Ectoine added to cDMEM media, promoted cell survival plus osteogenic and fibroblastic differentiation of hPDLMSCs.