Characterization of a novel Gram‐stain‐positive anaerobic coccus isolated from the female genital tract: Genome sequence and description of Murdochiella vaginalis sp. nov. Issue 3 (9th May 2018)

• Record Type: Journal Article
• Authors: Diop, Khoudia; Diop, Awa; Khelaifia, Saber; Robert, Catherine; Pinto, Fabrizio Di; Delerce, Jérémy; Raoult, Didier; Fournier, Pierre‐Edouard; Bretelle, Florence; Fenollar, Florence
• Is Part Of: MicrobiologyOpen. Volume 7:Issue 3(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-05-09
• Subjects: bacterial vaginosis -- culturomics -- genome -- Murdochiella vaginalis -- taxono‐genomics -- vaginal microbiota; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.570
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: Strain Marseille‐P2341 T, a nonmotile, nonspore‐forming, Gram‐stain‐positive anaerobic coccus, was isolated in the vaginal specimen of a patient with bacterial vaginosis using culturomics. Its growth occurred at temperatures ranging from 25 to 42°C, with pH between 6.5 and 8.5, and at NaCl concentrations lower than 5%. The major fatty acids were C18:1n9 (27.7%) and C16:0 (24.4%). Its genome is 1, 671, 491 bp long with 49.48 mol% of G+C content. It is composed of 1, 501 genes: 1, 446 were protein‐coding genes and 55 were RNAs. Strain Marseille‐P2341 T shared 97.3% of 16S rRNA gene sequence similarity with Murdochiella asaccharolytica, the phylogenetically closest species. These results enabled the classification of strain Marseille‐P2341 T as a new species of the genus Murdochiella for which we proposed the name Murdochiella vaginalis sp. nov. The type strain is strain Marseille‐P2341 T (=DSM 102237, =CSUR P2341). Abstract : Murdochiella vaginalis is a new bacterial species isolated in a vaginal sample from a patient with bacterial vaginosis. Murdochiella vaginalis is a Gram‐positive coccus‐shaped, nonmotile, and strictly anaerobic bacterium. The genome of M. vaginalis which 1, 671, 491 bp long contains 49.48 mol% G+C, 1, 446 protein‐coding genes and 55 RNAs.