Comparison of migration disturbance potency of epigallocatechin gallate (EGCG) synthetic analogs and EGCG PEGylated PLGA nanoparticles in rat neurospheres. (January 2019)

• Record Type: Journal Article
• Authors: Kühne, Britta A.; Puig, Teresa; Ruiz-Martínez, Santiago; Crous-Masó, Joan; Planas, Marta; Feliu, Lidia; Cano, Amanda; García, Maria Luisa; Fritsche, Ellen; Llobet, Joan M.; Gómez-Catalán, Jesús; Barenys, Marta
• Is Part Of: Food and chemical toxicology. Volume 123(2019)
• Page Start: 195
• Page End: 204
• Publication Date: 2019-01
• Subjects: Food supplements -- Neural progenitor cells -- Migration -- Neurospheres -- Developmental neurotoxicity; CEEA Ethic Committee for Animal Experimentation -- cMINC circular migration inhibition of neural crest cells -- CTB CellTiter-Blue Reagent -- DMEM Dulbecco's Modified Eagle Medium -- DMSO dimethyl sulfoxide -- DNT developmental neurotoxicity -- EC50 50% effective concentration -- EE encapsulation efficiency -- EGC epigallocatechin -- EGCG epigallocatechin gallate -- EGF epidermal growth factor -- FASN fatty acid synthase -- rrFGF rat recombinant fibroblast growth factor -- G37 1, 4-bis[(3, 4, 5-trihydroxybenzoyl)oxy]naphthalene -- G56 4, 4′-bis[(3, 4, 5-trihydroxybenzoyl)oxy]-1, 1′-biphenyl -- M1 4-hydroxy-2-naphthyl 3, 4, 5-trihydroxybenzoate -- M2 3-hydroxy-1-naphthyl 3, 4, 5-trihydroxybenzoate -- Nano-EGCG EGCG PEGylated-PLGA nanoparticles -- NPCs neural progenitor cells -- PBS phosphate buffered saline -- PDL poly-D-lysine -- PEG polyethylene glycol -- PEGylated-PLGA nanoparticles polyethylene glicosylated poly (lactic-co-glycolic acid) -- PFA paraformaldehyde -- PI polydispersity index -- PLGA poly (lactic-co-glycolic acid) -- PLGA-PEG poly (lactic-co-glycolic acid)-polyethylene glicol -- PND1 postnatal day 1 -- Zav average particle size -- ZP zeta potential; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.10.055
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Epigallocatechin gallate (EGCG), the main catechin of green tea, is described to have potential health benefits in several fields like oncology, neurology or cardiology. Currently, it is also under pre-clinical investigation as a potential therapeutic or preventive treatment during pregnancy against developmental adverse effects induced by toxic substances. However, the safety of EGCG during pregnancy is unclear due to its proven adverse effects on neural progenitor cells' (NPCs) migration. As lately several strategies have arisen to generate new therapeutic agents derived from EGCG, we have used the rat 'Neurosphere Assay' to characterize and compare the effects of EGCG structurally related compounds and EGCG PEGylated PLGA nanoparticles on a neurodevelopmental key event: NPCs migration. Compounds structurally-related to EGCG induce the same pattern of NPCs migration alterations (decreased migration distance, decreased formation of migration corona, chaotic orientation of cellular processes and decreased migration of neurons at higher concentrations). The potency of the compounds does not depend on the number of galloyl groups, and small structure variations can imply large potency differences. Due to their lower toxicity observed in vitro in NPCs, 4, 4′-bis[(3, 4, 5-trihydroxybenzoyl)oxy]-1, 1′-biphenyl and EGCG PEGylated PLGA nanoparticles are suggested as potential future therapeutic or preventive alternatives to EGCG during prenatal period. Highlights: Several compounds structurally-related to EGCG can induce the same pattern of alterations of NPCs migration than EGCG. The potency of the effects of these compounds does not depend on the inclusion of one or two galloyl groups. Within the tested compounds, small structure variations implied big potency differences in migration disturbance. G56 and Nano-EGCG are suggested to be evaluated as therapeutic or preventive alternatives to EGCG for the prenatal period.