In vitro and in vivo anti-leukemia activity of the stem bark of Salacia impressifolia (Miers) A. C. Smith (Celastraceae). (1st March 2019)

• Record Type: Journal Article
• Authors: Rodrigues, Ana Carolina B. da C.; Oliveira, Felipe P. de; Dias, Rosane B.; Sales, Caroline B.S.; Rocha, Clarissa A.G.; Soares, Milena B.P.; Costa, Emmanoel V.; Silva, Felipe M.A. da; Rocha, Waldireny C.; Koolen, Hector H.F.; Bezerra, Daniel P.
• Is Part Of: Journal of ethnopharmacology. Volume 231(2019)
• Page Start: 516
• Page End: 524
• Publication Date: 2019-03-01
• Subjects: 5-FU 5-fluorouracil -- ANOVA analysis of variance -- ATCC American Type Culture Collection -- CC column chromatography -- CTL negative control -- DMSO dimethyl sulfoxide -- DOX doxorubicin -- EAE ethyl acetate extract -- FEAE.3 fraction of the ethyl acetate extract -- FME.1 fraction of the methanol extract -- HE hexane extract -- HPLC high-performance liquid chromatography -- IC50 half maximal inhibitory concentration -- INPA National Institute of Research in the Amazon -- IR infrared -- ME methanol extract -- NMR nuclear magnetic resonance -- PBMC peripheral blood mononuclear cells -- SCID severe combined immunodeficient -- UV ultraviolet; 22-hydroxytingenone -- 5-fluorouracil -- Doxorubicin -- Pristimerin -- Tingenone; Salacia impressifolia -- Celastraceae -- Anti-leukemia -- HL-60; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2018.11.008
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Salacia impressifolia (Miers) A. C. Smith (family Celastraceae) is a traditional medicinal plant found in the Amazon Rainforest known as "miraruíra", "cipó-miraruíra" or "panu" and is traditionally used to treat dengue, flu, inflammation, pain, diabetes, male impotency, renal affections, rheumatism and cancer. Aim of the study: The aim of this study was to investigate in vitro and in vivo anti-leukemia activity of the stem bark of S. impressifolia in experimental models. Materials and methods: The in vitro cytotoxic activity of extracts, fractions and quinonemethide triterpenes (22-hydroxytingenone, tingenone and pristimerin) from the stem bark of S. impressifolia in cultured cancer cells was determined. The in vivo antitumor activity of the ethyl acetate extract (EAE) and of its fraction (FEAE.3) from the stem bark of S. impressifolia was assessed in C.B-17 severe combined immunodeficient (SCID) mice engrafted with human promyelocytic leukemia HL-60 cells. Results: The extract EAE, its fraction FEAE.3, and quinonemethide triterpenes exhibited potent cytotoxicity against cancer cell lines, including in vitro anti-leukemia activity against HL-60 and K-562 cells. Moreover, extract EAE and its fraction FEAE.3 inhibited the in vivo development of HL-60 cells engrafted in C.B-17 SCID mice. Tumor mass inhibition rates were measured as 40.4% and 81.5% for the extract EAE (20 mg/kg) and for its fraction FEAE.3 (20 mg/kg), respectively. Conclusions: Ethyl acetate extract and its fraction from the stem bark of S. impressifolia exhibit in vitro and in vivo anti-leukemia activity that can be attributed to their quinonemethide triterpenes. These data confirm the ethnopharmacological use of this species and may contribute to the development of a novel anticancer herbal medicine. Graphical abstract: