MicroRNA‐590‐5p functions as a tumor suppressor in breast cancer conferring inhibitory effects on cell migration, invasion, and epithelial–mesenchymal transition by downregulating the Wnt–β‐catenin signaling pathway. Issue 2 (7th September 2018)

• Record Type: Journal Article
• Authors: Gao, Jin; Yu, Shao‐Rong; Yuan, Yuan; Zhang, Li‐Li; Lu, Jian‐Wei; Feng, Ji‐Feng; Hu, Sai‐Nan
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 2(2019:Feb.)
• Page Start: 1827
• Page End: 1841
• Publication Date: 2018-09-07
• Subjects: breast cancer -- epithelial–mesenchymal transition -- invasion -- microRNA‐590‐5p -- migration -- PITX2 -- proliferation -- Wnt–β‐catenin signaling pathway; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27056
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Breast cancer remains one of the foremost primary causes of female morbidity and mortality worldwide. During the current study, the effect of miR‐590‐5p and paired‐like homeodomain transcription factor 2 (PITX2) on proliferation, invasion, migration, and epithelial–mesenchymal transition (EMT) of human breast cancer via the Wnt–β‐catenin signaling pathway was investigated. Breast cancer–related genes and related signaling pathways were obtained from KEGG database. The PITX2 regulatory microRNA was predicted. To define the contributory role by which miR‐590‐5p influences the progression of breast cancer, the interaction between miR‐590‐5p and PITX2 was explored; the proliferation, invasion, and migration abilities as well as the tumor growth and metastasis in nude mice were detected following the overexpression or silencing of miR‐590‐5p. PITX2 was determined to share a correlation with breast cancer and miR‐590‐5p was selected for further analysis. PITX2, Wnt‐1, β‐catenin, N‐cadherin, and vimentin all displayed higher levels, while miR‐590‐5p and E‐cadherin expression were lower among breast cancer tissues than in the adjacent normal tissue. After overexpression of miR‐590‐5p or si‐PITX2, the expression of E‐cadherin was markedly increased, decreases in the expression of Wnt‐1, β‐catenin, N‐cadherin, and vimentin, as well as inhibited cell proliferation, invasion, migration, metastasis, and EMT were observed. This study provides evidence suggesting that the transfection of overexpressed miR‐590‐5p can act to alleviate the effects of breast cancer demonstrating an ability to inhibit the processes of cell proliferation, migration, and invasion as well as EMT by suppressing the expression of PITX2 and activation of the Wnt–β‐catenin pathway. Abstract : This study provides evidence suggesting that the transfection of overexpressed miR‐590‐5p can act to alleviate the effects of breast cancer demonstrating an ability to inhibit the processes of cell proliferation, migration, and invasion as well as epithelial–mesenchymal transition by suppressing the expression of paired‐like homeodomain transcription factor 2 and activation of the Wnt–β‐catenin pathway.