Imaging of intracellular-specific microRNA in tumor cells by symmetric exponential amplification-assisted fluorescence in situ hybridization. Issue 99 (27th November 2018)

• Record Type: Journal Article
• Authors: Chen, Jun; Yin, Wen; Ma, Yingjun; Yang, Huihui; Zhang, Yanfei; Xu, Mengfei; Zheng, Xuling; Dai, Zong; Zou, Xiaoyong
• Is Part Of: Chemical communications. Volume 54:Issue 99(2018)
• Page Start: 13981
• Page End: 13984
• Publication Date: 2018-11-27
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc08849g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A symmetric exponential amplification-assisted fluorescence in situ hybridization (SEXPAR-FISH) strategy was reported for imaging intracellular-specific microRNAs. Abstract : We report a symmetric exponential amplification-assisted fluorescence in situ hybridization (SEXPAR-FISH) strategy for imaging intracellular-specific microRNAs (miRNAs) in cells. The strategy eliminates the risk of cell loss and miRNA leakage, and low-abundant intracellular miR-27a in cells can be imaged in only 2 h without compromising sensitivity and specificity.