The edible mushroom Albatrellus ovinus contains a α-l-fuco-α-d-galactan, α-d-glucan, a branched (1 → 6)-β-d-glucan and a branched (1 → 3)-β-d-glucan. (1st January 2019)

• Record Type: Journal Article
• Authors: Samuelsen, Anne Berit C.; Rise, Frode; Wilkins, Alistair L.; Teveleva, Lioubov; Nyman, Anna Armika Tussilago; Aachmann, Finn L.
• Is Part Of: Carbohydrate research. Volume 471(2019)
• Page Start: 28
• Page End: 38
• Publication Date: 2019-01-01
• Subjects: Albatrellus ovinus -- Fucogalactan -- β-glucan -- α-glucan; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2018.10.012
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material. Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide composition, glycosidic linkages by methylation and GC-MS as well as NMR spectroscopy. A fucogalactan composed of an (1 → 6)-α-d -galactan backbone with single α-l -Fuc p residues attached at O -2 was identified in the hot water extract obtained after treatment with a protease and size exclusion chromatography. Both the hot water extract and the hot alkali extract contained an (1 → 4)-α-d -glucan whereas β-d -glucans were mainly present in the latter. Structural analysis suggested the presence of two different β-d -glucan backbone structures; a (1 → 6)-linked β-d -glucan with single β-d -Glc p residues at O -3 and also a (1 → 3)-linked β-d -glucan with branches in O-6. In addition there were identified short (1 → 2)-linked β-d -xylan and (1 → 3)-α-d -mannan chains. Graphical abstract: Highlights: The sheep polypore ( Albatrellus ovinus ) is considered an edible mushroom. A.ovinus contains at least four different extractable cell wall polysaccharides. The water soluble polysaccharides includes a fucogalactan and a glycogen-like α-d -glucan. A.ovinus contains a branched (1. → 6)-β-d -glucan and (1 → 3)-linked β-d -glucan in addition to chains of α-d -mannan and β-d -xylan