Clonorchis sinensis omega-class glutathione transferases are reliable biomarkers for serodiagnosis of clonorchiasis and opisthorchiasis. (January 2019)

• Record Type: Journal Article
• Authors: Kim, J.-G.; Ahn, C.-S.; Sripa, B.; Eom, K.S.; Kang, I.; Sohn, W.-M.; Nawa, Y.; Kong, Y.
• Is Part Of: Clinical microbiology and infection. Volume 25:Number 1(2019)
• Page Start: 109.e1
• Page End: 109.e6
• Publication Date: 2019-01
• Subjects: Clonorchis sinensis -- Omega-class glutathione transferase -- Opisthorchiidae -- Opisthorchis viverrini -- Serodiagnostic biomarker; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1016/j.cmi.2018.03.042
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: Objectives: To determine the potential for immunodiagnostic application of two recombinant forms of Clonorchis sinensis omega-class glutathione transferases (rCsGSTo1 and rCsGSTo2) against human small liver-fluke C. sinensis and Opisthorchis viverrini infections. Methods: Specific antibody levels against rCsGSTo1 and rCsGSTo2 in patients' sera of egg-positive opisthorchiasis ( n = 87) and clonorchiasis ( n = 120), as well as those in sera from patients with other helminthic infections ( n = 252) and healthy controls ( n = 40) were retrospectively analysed by ELISA. Results: We observed highly positive correlation coefficients between specific antibody levels against rCsGSTo1 and rCsGSTo2 and egg counts per gramme of faeces (EPG) of patients with opisthorchiasis ( n = 87; r = 0.88 for rCsGSTo1 and r = 0.90 for rCsGSTo2). Sera from opisthorchiasis patients whose EPG counts >100 ( n = 43) revealed high antibody titres against both antigens. Patients' sera with low EPG counts (<100, n = 44) also exhibited reliable sensitivities of 93.2% and 97.7% for rCsGSTo1 and rCsGSTo2, respectively. Sera from clonorchiasis patients showed sensitivities of 90% (108/120 samples) and 89.2% (107/120 sera) for rCsGSTo1 and rCsGSTo2. Overall diagnostic sensitivities for liver-fluke infections were 92.3% for rCsGSTo1 (191/207 samples) and 93.2% for rCsGSTo2 (193/207 samples). Specificities were 89.7% (rCsGSTo1) and 97.6% (rCsGSTo2). Conclusions: Detection of specific antibody levels against rCsGSTo1 or rCsGSTo2 might be promising for the serodiagnosis of patients infected with these two phylogenetically close carcinogenic liver-flukes.