A near-infrared fluorescent probe for amyloid-β aggregates. (March 2019)

• Record Type: Journal Article
• Authors: Lee, Misun; Kim, Mingeun; Tikum, Anjong Florence; Lee, Hyuck Jin; Thamilarasan, Vijayan; Lim, Mi Hee; Kim, Jinheung
• Is Part Of: Dyes and pigments. Volume 162(2019)
• Page Start: 97
• Page End: 103
• Publication Date: 2019-03
• Subjects: Amyloid-β -- Fluorescent probe -- Dimethylaminostyrene -- Benzo[e]indole -- Donor-π-acceptor system; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2018.10.013
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: The deposition of amyloid-β (Aβ) aggregates in the brain is a hallmark of the Alzheimer's disease (AD)-affected brain. Various aggregated Aβ species, including oligomers and fibrils, are generated upon the aggregation process, and these Aβ aggregates have distinct biological properties. To non-invasively monitor the aggregation pathways of Aβ and identify the existence of certain Aβ species in biological environments, an effective strategy to detect Aβ species is necessary. Herein, we report a turn-on near-infrared (near-IR) fluorescent probe, 1, for Aβ aggregates, which consists of a donor-π-acceptor system with an Aβ-interacting moiety. Our probe, 1, shows a noticeable increase in near-IR fluorescence at ca. 710 nm in the presence of Aβ aggregates in aqueous media. In addition, the fluorescent response of1 was altered depending on the degree of Aβ aggregation. Moreover, 1 indicates turn-on fluorescence with Aβ aggregates in living cells and is nontoxic under the condition used for live-cell imaging. Graphical abstract: Highlights: A near-IR fluorescent probe for detecting Aβ species was developed. 1 monitors Aβ aggregation with a change in fluorescence in aqueous media. 1 shows turn-on fluorescence with Aβ species in living cells.