Kinesin-5 Regulation and Function in Mitosis. Issue 1 (January 2019)

• Record Type: Journal Article
• Authors: Mann, Barbara J.; Wadsworth, Patricia
• Is Part Of: Trends in cell biology. Volume 29:Issue 1(2019)
• Page Start: 66
• Page End: 79
• Publication Date: 2019-01
• Subjects: mitosis -- Eg5 -- kinesin-5 -- mitotic spindle; Cytology -- Periodicals; Cytology -- Research -- Periodicals; 571.6
• DOI: 10.1016/j.tcb.2018.08.004
• Languages: English
• ISSNs: 0962-8924

Abstract

Abstract : Chromosome segregation during cell division requires a bipolar mitotic spindle. Therefore, how the spindle is formed, maintained, and functions is of fundamental importance for all eukaryotic cells. Members of the evolutionarily conserved kinesin-5 family of motor proteins have been shown to play an essential role in spindle formation by generating forces that establish and maintain spindle bipolarity and contribute to spindle elongation. Recent work demonstrates that accessory proteins and post-translational modifications regulate the localization and activity of kinesin-5 motors in cells. In addition, some kinesin-5 motors can move toward the microtubule plus-or-minus end. This new information provides insight into how these motors function during mitosis. Highlights: Kinesin-5 motors are phosphorylated at multiple sites within the protein; phosphorylation impacts motor activity, localization, and function in mitosis. Kinesin-5 motors walk toward the microtubule plus end. In yeast, kinesin-5 additionally shows minus-end-directed motion that affects mitosis. Localization of vertebrate kinesin-5 motors to spindle microtubules and the centrosome requires a microtubule-associated protein, TPX2. Spindle bipolarity requires kinesin-5 activity in diverse cells; in some cases kinesin-12 can substitute for kinesin-5 and drive spindle formation.