Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata. (March 2019)

• Record Type: Journal Article
• Authors: Pinaud, Silvain; Portet, Anaïs; Allienne, Jean-François; Belmudes, Lucid; Saint-Beat, Cécile; Arancibia, Nathalie; Galinier, Richard; Du Pasquier, Louis; Duval, David; Gourbal, Benjamin
• Is Part Of: Developmental and comparative immunology. Volume 92(2019)
• Page Start: 238
• Page End: 252
• Publication Date: 2019-03
• Subjects: Immunological memory -- Specificity -- Schistosoma -- Biomphalaria -- RNAseq -- Proteomic -- Vaccination; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2018.12.001
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Invertebrate immune response may be primed by a current infection in a sustained manner, leading to the failure of a secondary infection with the same pathogen. The present study focuses on the Schistosomiasis vector snail Biomphalaria glabrata, in which a specific genotype-dependent immunological memory was demonstrated as a shift from a cellular to a humoral immune response. Herein, we investigate the complex molecular bases associated with this genotype-dependant immunological memory response. We demonstrate that Biomphalaria regulates a polymorphic set of immune recognition molecules and immune effector repertoires to respond to different strains of Schistosoma parasites. These results suggest a combinatorial usage of pathogen recognition receptors (PRRs) that distinguish different strains of parasites during the acquisition of immunological memory. Immunizations also show that snails become resistant after exposure to parasite extracts. Hemolymph transfer and a label-free proteomic analysis proved that circulating hemolymph compounds can be produced and released to more efficiently kill the newly encountered parasite of the same genetic lineage. Graphical abstract: Highlights: Immunological memory (IM) has been now demonstrated in numerous invertebrate models. A high level of specificity of IM has been demonstrated in Biomphalaria glabrata snails. Efficiency of IM seems to be supported by a potential combinatorial usage of PRRs. PRRs and cytolytic/cytotoxic compounds were mandatory for parasite killing.