Amido Black 10B a widely used azo dye causes DNA damage in pro- and eukaryotic indicator cells. (February 2019)

• Record Type: Journal Article
• Authors: Carvalho da Cruz Brambilla, Crislaine Maria; Hilario Garcia, Ana Leticia; Rabaioli da Silva, Fernanda; Taffarel, Silvio Roberto; Grivicich, Ivana; Picada, Jaqueline Nascimento; Scotti, Amanda; Dalberto, Daiana; Mišík, Miroslav; Knasmüller, Siegfried; da Silva, Juliana
• Is Part Of: Chemosphere. Volume 217(2019)
• Page Start: 430
• Page End: 436
• Publication Date: 2019-02
• Subjects: Amido black 10B -- Azo dye -- Cytotoxicity -- Genotoxicity -- Mutagenicity; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.11.026
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Acid Black 10B (AB10B) is widely used for the production of textiles, leather and prints. It is a representative of azo dyes and it is well documented that some of these compounds are mutagenic per se, and that cleavage products (in particular aromatic amines) may cause damage of the genetic material and cancer. Since no toxicological data on AB10B have been published, we evaluated its mutagenic activity in Salmonella/microsome assays and studied its acute toxic and genotoxic properties in a human derived liver cell line (HepG2) which retained the activities of drug metabolizing enzymes. The compound did not cause cytotoxicity (MTT assay), but clear genotoxic effects were detected in pro- and eukaryotic indicator cells. Dose dependent induction of his + revertants was seen in strain TA98 which detects frameshift mutations without metabolic activation; a more pronounced effect was seen in its derivative YG1024 which overexpresses N-acetyltransferase. Induction of single/double strand breaks by Comet assay was detected with concentrations > 0.125 mg/mL in liver derived cells; as well as increased rates for micronucleus (reflecting structural and numeric chromosomal aberrations) and nuclear buds which are a consequence of gene amplifications were seen with a higher dose (2.0 mg/mL) (p < 0.05; Tukey's test). The mutational pattern which was observed in the bacterial tests indicates that the cleavage product p -nitroaniline may cause the genotoxic effects of the dye. Our findings indicate that exposure of humans and the release of the compound into the environment may lead to adverse effects due to its DNA damaging activity. Highlights: Amido Black 10B causes genotoxic effect in HepG2 cells (comet and micronucleus tests). Amido Black 10B causes mutagenic activity in Salmonella/microsome assay. Genotoxic effects of Amido Black 10B can be related with the product p -nitroaniline.