Genotoxic action of naphthenic acids on the fish macrophage cell line, RTS11. (2018)

• Record Type: Journal Article
• Authors: Trento, Marcus Vinicius Cardoso; César, Pedro Henrique Souza; Marcussi, Silvana; Lee, Lucy E.J.
• Is Part Of: International journal of environment and pollution. Volume 63:Number 1/2(2018)
• Page Start: 117
• Page End: 130
• Publication Date: 2018
• Subjects: environmental pollution -- DNA fragmentation -- comet assay -- rainbow trout -- naphthenic acids -- NAs -- RTS11 cell line; Environmental policy -- Periodicals; Environmental engineering -- Periodicals; Environmental sciences -- Periodicals; Pollution -- Periodicals; 363.73
• Languages: English
• ISSNs: 0957-4352

Abstract

Naphthenic acids (NAs) are chemical constituents found in oil sands process-affected waters (OSPW) that are distinctly toxic to biota. They comprise an ill-defined mixture of carboxylic acids implicated in endocrine disruption, acute toxicity, immunotoxicity, tissue malformation and embryotoxicity in several fish species, in addition to being cytotoxic. Recent evidence indicates a possible genotoxic effect and the present study evaluated the genotoxic potential of NAs using the 'comet' assay and the rainbow trout spleen cell line, RTS11. The results provided a genotoxic value (in arbitrary units) two times higher for the NA concentration at 10 μg/mL (≈ 105) relative to the NA at 1 μg/mL (≈ 45). NAs at 10 μg/mL gave a damage index of 45.86% compared to the damage observed with a positive control-hydrogen peroxide, which was 90.66% (evaluated at 100 μM). The data obtained suggest a genotoxic potential for NAs at doses that could occur naturally in OSPW.