A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata. (February 2018)

• Record Type: Journal Article
• Authors: Williams, Leah M.; Fuess, Lauren E.; Brennan, Joseph J.; Mansfield, Katelyn M.; Salas-Rodriguez, Erick; Welsh, Julianne; Awtry, Jake; Banic, Sarah; Chacko, Cecilia; Chezian, Aarthia; Dowers, Donovan; Estrada, Felicia; Hsieh, Yu-Hsuan; Kang, Jiawen; Li, Wanwen; Malchiodi, Zoe; Malinowski, John; Matuszak, Sean; McTigue, Thomas; Mueller, David; Nguyen, Brian; Nguyen, Michelle; Nguyen, Phuong; Nguyen, Sinead; Njoku, Ndidi; Patel, Khusbu; Pellegrini, William; Pliakas, Tessa; Qadir, Deena; Ryan, Emma; Schiffer, Alex; Thiel, Amber; Yunes, Sarah A.; Spilios, Kathryn E.; Pinzón C, Jorge H.; Mydlarz, Laura D.; Gilmore, Thomas D.;
• Is Part Of: Developmental and comparative immunology. Volume 79(2018)
• Page Start: 128
• Page End: 136
• Publication Date: 2018-02
• Subjects: NF-kappaB -- Toll-like receptor -- Evolution -- Coral -- Innate immunity -- Orbicella faveolata; IKK IkappaB kinase -- LRR leucine-rich repeat domain -- Nv Nematostella vectensis -- NF-κB nuclear factor kappa B -- Of Orbicella faveolata -- TIR Toll/interleukin-1 receptor -- TLR Toll-like Receptor -- TM transmembrane; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.10.016
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Herein, we characterize the Toll-like receptor (TLR)-to-NF-κB innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-κB pathway mobilization. Biochemical and cell-based assays revealed that Of-NF-κB resembles the mammalian non-canonical NF-κB protein p100 in that C-terminal truncation results in translocation of Of-NF-κB to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human IκB kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-κB in vitro and induce C-terminal processing of Of-NF-κB in vivo . These results are the first characterization of TLR-to-NF-κB signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways. Highlights: A Toll-like receptor to NF-κB pathway is present in the coral Orbicella faveolata. Activities of O. faveolata TLR and NF-κB proteins are characterized. LPS treatment of coral tissue activated an NF-κB gene expression profile.