A Solid‐State Hard Microfluidic–Nanopore Biosensor with Multilayer Fluidics and On‐Chip Bioassay/Purification Chamber. (16th October 2018)

• Record Type: Journal Article
• Authors: Varongchayakul, Nitinun; Hersey, Joseph S.; Squires, Allison; Meller, Amit; Grinstaff, Mark W.
• Is Part Of: Advanced functional materials. Volume 28:Number 50(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-16
• Subjects: DNA purification -- microfluidics -- nanopore; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201804182
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Solid‐state nanopores are an emerging biosensor for nucleic acid and protein characterization. For use in a clinical setting, solid‐state nanopore sensing requires sample preparation and purification, fluid handling, a heating element, electrical noise insulators, and an electrical readout detector, all of which hamper its translation to a point‐of‐care diagnostic device. A stand‐alone microfluidic‐based nanopore device is described that combines a bioassay reaction/purification chamber with a solid‐state nanopore sensor. The microfluidic device is composed of the high‐temperature/solvent resistance Zeonex plastic, formed via micromachining and heat bonding, enabling the use of both a heat regulator and a magnetic controller. Fluid control through the microfluidic channels and chambers is controlled via fluid port selector valves and allows up to eight different solutions. Electrical noise measurements and DNA translocation experiments demonstrate the integrity of the device, with performance comparable to a conventional stand‐alone nanopore setup. However, the microfluidic–nanopore setup is superior in terms of ease of use. To showcase the utility of the device, single molecule detection of a DNA polymerase chain reaction product, after magnetic bead DNA separation, is accomplished on‐chip. Abstract : A combined microfluidic and solid‐state nanopore device enables facile single bio‐macromolecule detection. The device is composed of Zeonex plastic, and features fluid handling, a heating element, and a chamber for bioassay reactions and purifications. Device performance specifications are comparable to a conventional stand‐alone nanopore setup, and the device successfully identifies a DNA polymerase chain reaction product, after separation on‐chip.