PEGylated DOTA‐AHA‐Based GdIII Chelates: A Relaxometric Study. Issue 29 (4th September 2015)

• Record Type: Journal Article
• Authors: Fontes, André; Karimi, Shima; Helm, Lothar; Ferreira, Paula M.; André, João P.
• Is Part Of: European journal of inorganic chemistry. Issue 29(2015)
• Page Start: 4798
• Page End: 4809
• Publication Date: 2015-09-04
• Subjects: Macrocyclic ligands -- Ligand design -- Lanthanides -- Chelates -- Imaging agents; Chemistry, Inorganic -- Periodicals; Organometallic chemistry -- Periodicals; Bioinorganic chemistry -- Periodicals; Solid state chemistry -- Periodicals; 546
• DOI: 10.1002/ejic.201500688
• Languages: English
• ISSNs: 1434-1948

Abstract

Abstract: Three PEGylated derivatives of 6‐amino‐2‐[4, 7, 10‐tris(carboxymethyl)‐1, 4, 7, 10‐tetrazacyclododec‐1‐yl]hexanoic acid (DOTA‐AHA) with different molecular weights were prepared and characterized. Their Gd III chelates were studied in aqueous solution through variable‐temperature 1 H nuclear magnetic relaxation dispersion (NMRD) and 17 O NMR spectroscopy to determine their relaxivities and the parameters that govern them. The relaxivity varied from 5.1 to 6.5 mM –1 s –1 (37 °C and 60 MHz) as the molecular weight of the polyethylene glycol (PEG) chain increased and was slightly higher than that of the parent chelate Gd(DOTA‐AHA), owing to a small contribution of a slow global rotation of the complexes. A variable‐temperature 1 H NMR spectroscopy study of several Ln III chelates of DOTA‐A(PEG750 )HA allowed the determination of the isomeric M / m ratio ( M = square‐antiprismatic isomer and m = twisted square‐antiprismatic isomer; the latter presents a much faster water exchange), which was estimated to be ca. 1:0.2 for the Gd III chelate, very close to that of [Gd(DOTA)] – . This explains why the PEGylated Gd III chelate has a water rate exchange similar to that of [Gd(DOTA)] – . The predominance of the M isomer is a consequence of the bulky PEG moiety, which does not favor the stabilization of the m isomer in sterically crowded systems at the substituent site, in contrast to what happens with less packed asymmetrical DOTA‐type chelates with substitution at one of the four acetate C( α ) atoms. Abstract : Three PEGylated derivatives of DOTA‐AHA with different molecular weights are prepared, and their Gd III chelates are studied by variable‐temperature 1 H nuclear magnetic relaxation dispersion (NMRD) and 17 O NMR spectroscopy. The isomeric M / m ratio is determined by a 1 H NMR study of several Ln III chelates of DOTA‐A(PEG750 )HA to shed light on the relaxometric behavior of the Gd III chelates.