Detection of biogenic amines in pet food ingredients by RP‐HPLC with automated dansyl chloride derivatization. Issue 24 (2nd November 2018)

• Record Type: Journal Article
• Authors: Learey, Jessica J.; Crawford‐Clark, Shona; Bowen, Benjamin J.; Barrow, Colin J.; Adcock, Jacqui L.
• Is Part Of: Journal of separation science. Volume 41:Issue 24(2018)
• Page Start: 4430
• Page End: 4436
• Publication Date: 2018-11-02
• Subjects: automation -- biogenic amines -- dansyl chloride -- derivatization -- pet food; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201800455
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: The detection of biogenic amines is of significant interest to the food industry, as they can be used as indicators of food spoilage and they are potentially toxic. Because of their importance, there is a need for automated methods suitable for industry use that can detect a wide range of biogenic amines at sufficient levels for food analysis. In this work, optimized conditions for the automated determination of biogenic amines (histamine, putrescine, cadaverine, spermine, spermidine, tyramine, and tryptamine) derivatized with dansyl chloride are presented. Limits of detection below 0.2 ppm were achieved for seven biogenic amines and percentage recoveries were between 80 and 109% for the seven analytes spiked into meat meal samples. The method is simple and compared well to an existing method for the detection of biogenic amines in pet food ingredients.