From single-molecule spectroscopy to super-resolution imaging of the neuron: a review. (24th June 2016)

• Record Type: Journal Article
• Authors: Laine, Romain F; Kaminski Schierle, Gabriele S; van de Linde, Sebastian; Kaminski, Clemens F
• Is Part Of: Methods and applications in fluorescence. Volume 4:Number 2(2016:Jun.)
• Publication Date: 2016-06-24
• Subjects: single-molecule localization microscopy -- neurodegeneration -- neuroscience -- bioimaging -- super-resolution microscopy -- protein misfolding and aggregation; Fluorescence spectroscopy -- Periodicals; Fluorescence -- Periodicals; Spectrometry, Fluorescence -- Periodicals; Fluorescence -- Periodicals; Fluorescence; Fluorescence spectroscopy; Periodicals; 535.352
• DOI: 10.1088/2050-6120/4/2/022004
• Languages: English
• ISSNs: 2050-6120

Abstract

Abstract: For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.