Isolongifolanone-based molecular fluorescence marker for imaging endogenous Zn2+ in vivo. Issue 40 (5th October 2017)

• Record Type: Journal Article
• Authors: Wang, Zhonglong; Yang, Jinlai; Liu, Pengcheng; Yang, Yiqin; Fang, Hua; Xu, Xu; Rui, Jian; Xu, Haijun; Wang, Shifa
• Is Part Of: Tetrahedron. Volume 73:Issue 40(2017)
• Page Start: 5912
• Page End: 5919
• Publication Date: 2017-10-05
• Subjects: Isolongifolanone -- Rapid response -- Concentration-dependence -- DFT calculation -- Bio-imaging endogenous Zn2+; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2017.08.033
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: A novel Zn 2+ -specific molecular probe was synthesized from renewable isolongifolanone. The probe emitted strong green fluorescence with high excitation and emission wavelengths induced by Zn 2+ and showed a high sensitivity towards Zn 2+ with a lower detection limit of 5.12 nM based on the fluorescence linearity relationship studies. The detection of the probe towards Zn 2+ ion exhibited a wide pH range (4–10) with a short response time and excellent anti-interference. Then, the structure-coordination of the probe towards Zn 2+ was determined by DFT calculations, and its concentration dependence on Zn 2+ was demonstrated on test papers. In addition, the probe was successfully used for imaging the local enriched Zn 2+ with a real-time system in vivo in living animals. Thus, the molecular fluorescence probe synthesized from isolongifolanone could potentially contribute to labelling endogenous Zn 2+ in living organisms. Graphical abstract: