Expressional divergences of two desaturase genes determine the opposite ratios of two sex pheromone components in Helicoverpa armigera and Helicoverpa assulta. (November 2017)

• Record Type: Journal Article
• Authors: Li, Rui-Ting; Ning, Chao; Huang, Ling-Qiao; Dong, Jun-Feng; Li, Xianchun; Wang, Chen-Zhu
• Is Part Of: Insect biochemistry and molecular biology. Volume 90(2017)
• Page Start: 90
• Page End: 100
• Publication Date: 2017-11
• Subjects: Helicoverpa armigera -- Helicoverpa assulta -- Sex pheromone gland transcriptome -- Desaturase -- Backcross -- Fatty acyl reductase; Insect biochemistry -- Periodicals; Insects -- Physiology -- Periodicals; Insects -- Molecular aspects -- Periodicals; Biochemistry -- Periodicals; Insectes -- Biochimie -- Périodiques; Insectes -- Composition -- Périodiques; Insectes -- Physiologie -- Périodiques; Insectes -- Aspect moléculaire -- Périodiques; Biochimie -- Périodiques; Biochemistry; Insect biochemistry; Insects -- Molecular aspects; Insects -- Physiology; Periodicals; 572.8157
• DOI: 10.1016/j.ibmb.2017.09.016
• Languages: English
• ISSNs: 0965-1748

Abstract

Abstract: The sympatric closely related species Helicoverpa armigera and Helicoverpa assulta use 97:3 and 7:93 of (Z)-11-hexadecenal and (Z)-9-hexadecenal, respectively, as their sex pheromone to find/locate correct sex mates. Moreover, (Z)-11-hexadecenyl alcohol and (Z)-9-hexadecenyl alcohol are more abundant in the pheromone gland of H. assulta than in that of H. armigera . To clarify the molecular basis of these differences, we sequenced the pheromone gland transcriptomes of the two species and compared the expression patterns of the candidate enzyme genes involved in the pheromone biosynthetic pathways by FPKM values and quantitative RT-PCR analysis. We found that the desaturase gene LPAQ expressed about 70 times higher in H. armigera than in H. assulta, whereas another desaturase gene NPVE expressed about 60 times higher in H. assulta than in H. armigera . We also observed significantly higher expression of the fatty acyl reductase (FAR) gene FAR1 and the aldehyde reductase (AR) gene AR3 in H. assulta than in H. armigera . Examination of the pheromone glands of the backcross offspring of their hybrids to H. assulta showed a positive linear correlation between the expression level of LPAQ and the amount of Z11-16:Ald and between the expression level of NPVE and the amount of Z9-16:Ald in the pheromone glands. Taken together, these data demonstrate that the expressional divergences of LPAQ and NPVE determine the opposite sex pheromone component ratios in the two species and the divergent expression of FAR1 and AR3 may account for the greater accumulation of alcohols in the pheromone gland of H. assulta . Graphical abstract: Highlights: 90 and 86 sex pheromone production related enzyme genes were identified in H. armigera and H. assulta respectively. Tissue expression patterns of all the identified enzyme genes were exhibited. LPAQ and NPVE determine the opposite ratios of the two sex pheromone components in the two species. HassFAR1 and HassAR3 may account for accumulation of alcohols in H. assulta.