A general gas‐assisted three‐liquid‐phase extraction method for separation and concentration of puerarin, 3′‐methoxydaidzin, puerarinxyloside, daidzin and daidzein from puerariae extract. (16th October 2018)

• Record Type: Journal Article
• Authors: Hu, Weilun; Shao, Qian; Xi, Xingjun; Chu, Qiao; Lan, Tao; Che, Fenfang; Liu, Yuanyuan; Lu, Yanzhen; Wei, Yun
• Other Names: Weng Naidong guestEditor.; Srinivas Nuggehally guestEditor.
• Is Part Of: Biomedical chromatography. Volume 33:Number 1(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-16
• Subjects: gas‐assisted three‐liquid‐phase extraction -- isoflavonoids -- puerariae; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.4390
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: In this work, a general and novel separation technique gas‐assisted three‐liquid‐phase extraction was established and applied in separating and concentrating isoflavonoids from the actual sample of puerariae extract by one step. For the gas‐assisted three‐liquid‐phase extraction method, optimal conditions were selected: polyethylene glycol 2000 and ethyl acetate as the flotation solvent, pH 5, (NH4 )2 SO4 concentration 350 g/L in aqueous phase, N2 flow rate 30 mL/min, flotation time 50 min, and flotation twice. Five isoflavonoids compounds puerarin, 3′‐methoxydaidzin, puerarinxyloside, daidzin and daidzein were separated with recoveries of 82, 84, 80, 88 and 89%, respectively. The separated products were purified by preparative high‐performance liquid chromatography, and the purity of the final products was >96%. The established general gas‐assisted three‐liquid‐phase extraction was used to separate anthraquinones from Cassiae Semen under the optimal conditions, and the recoveries were >75%. The experimental results showed that the established gas‐assisted three‐liquid‐phase extraction method is a general technique for separating active compounds from herb extract.