Purification, characterization and biological effect of reversing the kidney-yang deficiency of polysaccharides from semen cuscutae. (1st November 2017)

• Record Type: Journal Article
• Authors: Yang, Song; Xu, Xinfang; Xu, Hefang; Xu, Shuya; Lin, Qinghua; Jia, Zhe; Han, Ting; Zhang, Hui; Zhang, Ying; Liu, Huan; Gao, Yan; Li, Xiangri
• Is Part Of: Carbohydrate polymers. Volume 175(2017)
• Page Start: 249
• Page End: 256
• Publication Date: 2017-11-01
• Subjects: Semen cuscutae -- Polysaccharides -- Kidney-yang deficiency -- Purification -- Characterization -- Mannose; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.07.077
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: The polysaccharides from semen cuscutae could reverse the kidney-yang deficiency. Three homogeneous polysaccharides were obtained which were named as C-7WR1, C-7WR2 and C-7WR3 by DEAE-cellulose and Sephacryl S-400. Their structures were studied by a variety of research methods. They all mainly contained mannose. Abstract: Semen cuscutae is a well-known Chinese medicine which has been used to nourish kidney. It is the first study to demonstrate that the polysaccharides from semen cuscutae showed significant activity of nourishing kidney-yang by increasing the levels of testosterone and estradiol, decreasing the level of blood urea nitrogen, improving immune function, possessing antioxidant effect. Three homogeneous polysaccharides were obtained by DEAE-cellulose and Sephacryl S-400 which were named as C-7WR1, C-7WR2 and C-7WR3 with average molecular weight of 7.59 × 10 4, 3.23 × 10 4 and 2.25 × 10 4 respectively. C-7WR1 was composed of fructose: mannose = 0.02:1. C-7WR2 was composed of fructose: mannose: xylose: arabinose = 0.01:1:0.14:0.33. C-7WR3 was composed of fructose: mannose: xylose: arabinose = 0.01:1:0.10:0.47. They mainly contained mannose. Their fourier transform infrared features were similar. They all had no nucleic acid and protein.