OsPRR37 confers an expanded regulation of the diurnal rhythms of the transcriptome and photoperiodic flowering pathways in rice. (5th February 2018)

• Record Type: Journal Article
• Authors: Liu, Chuan; Qu, Xuefeng; Zhou, Yanhao; Song, Gaoyuan; Abiri, Naghmeh; Xiao, Yuhui; Liang, Fan; Jiang, Daiming; Hu, Zhongli; Yang, Daichang
• Is Part Of: Plant, cell and environment. Volume 41:Number 3(2018)
• Page Start: 630
• Page End: 645
• Publication Date: 2018-02-05
• Subjects: circadian -- clock‐output genes; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.13135
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: The circadian clock enables organisms to rapidly adapt to the ever‐changing environmental conditions that are caused by daily light/dark cycles. Circadian clock genes universally affect key agricultural traits, particularly flowering time. Here, we show that OsPRR37, a circadian clock gene, delays rice flowering time in an expression level‐dependent manner. Using high‐throughput mRNA sequencing on an OsPRR37 overexpressing transgenic line ( OsPRR37 ‐OE5) and the recipient parent Guangluai4 that contains the loss‐of‐function Osprr37, we identify 14, 992 genes that display diurnal rhythms, which account for 52.9% of the transcriptome. Overexpressing OsPRR37 weakens the transcriptomic rhythms and alters the phases of rhythmic genes. In total, 3, 210 differentially expressed genes (DEGs) are identified, among which 1, 863 rhythmic DEGs show a correlation between the change of absolute amplitudes and the mean expression levels. We further reveal that OsPRR37 functions as a transcriptional repressor to repress the expression levels and amplitudes of day‐phased clock genes. More importantly, OsPRR37 confers expanded regulation on the evening‐phased rhythmic DEGs by repressing the morning‐phased rhythmic DEGs. Further study shows that OsPRR37 expands its regulation on flowering pathways by repressing Ehd1 . Thus, our results demonstrate an expanded regulation mechanism of the circadian clock on the diurnal rhythms of the transcriptome. Abstract : Circadian clock universally regulates the rhythms of gene transcription. Here, we show that rice circadian clock gene, OsPRR37, globally regulates gene transcription in the changing diurnal environment. OsPRR37 confers an expanded regulation of the evening‐phased rhythmic DEGs by repressing the morning‐phased rhythmic DEGs. Our results demonstrate an expanded regulation mechanism of the circadian clock on the diurnal rhythms of the transcriptome, which provides a potential way to improve rice agronomic traits by transcriptionally or genetically manipulating the circadian clock genes and clock‐output pathways.