MicroRNA-886-3P functions as a tumor suppressor in small cell lung cancer. Issue 12 (2nd December 2018)

• Record Type: Journal Article
• Authors: Shen, Jie; Zhou, Wei; Bi, Nan; Song, Yong-Mei; Zhang, Fu-Quan; Zhan, Qi-Min; Wang, Lv-Hua
• Is Part Of: Cancer biology & therapy. Volume 19:Issue 12(2018)
• Page Start: 1185
• Page End: 1192
• Publication Date: 2018-12-02
• Subjects: Small cell lung cancer -- miRNAs -- miR-886-3P -- TGF-ß1 -- aggressive disease -- fetal bovine serum -- pGL3-luciferase -- cancer biology; 616.99406
• DOI: 10.1080/15384047.2018.1491505
• Languages: English
• ISSNs: 1538-4047

Abstract

ABSTRACT: Small cell lung cancer (SCLC) is a highly aggressive disease and miRNAs may play an important role in modulating SCLC progression. We have previously screened 924 miRNAs and found that miR-886-3P was negatively associated with SCLC survival. In the current study, we further investigated the role of miR-886-3P mimic in regulating SCLC cell phenotypic alteration in vitro and xenograft tumor formation in vivo . We found that transfection of miR-886-3P mimic significantly inhibited SCLC cell proliferation, migration, and colony formation, and induced mesenchymal-epithelial transition (MET) by suppressing TGF-ß1 synthesis in vitro . Furthermore, intra-tumor injection of miR-886-3P mimic lead to necrosis and suppression of tumor invasion to the surrounding tissue in the subcutaneous xenograft tumor, and intra-vein injection of miR-886-3P mimic suppressed xenograft lung cancer growth in vivo . These findings suggested that miR-886-3P functions as a tumor suppressor in SCLC and thus, it might be a potential therapeutic molecule in the treatment of lung cancer.