Layer-by-Layer 3D Constructs of Fibroblasts in Hydrogel for Examining Transdermal Penetration Capability of Nanoparticles. (August 2017)

• Record Type: Journal Article
• Authors: Hou, Xiaochun; Liu, Shiying; Wang, Min; Wiraja, Christian; Huang, Wei; Chan, Peggy; Tan, Timothy; Xu, Chenjie
• Is Part Of: SLAS technology. Volume 22:Number 4(2017)
• Page Start: 447
• Page End: 453
• Publication Date: 2017-08
• Subjects: artificial skin model -- 3D printing -- hydrogel -- nanoparticles -- transdermal delivery; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2211068216655753
• Languages: English
• ISSNs: 2472-6303

Abstract

Nanoparticles are emerging transdermal delivery systems. Their size and surface properties determine their efficacy and efficiency to penetrate through the skin layers. This work utilizes three-dimensional (3D) bioprinting technology to generate a simplified artificial skin model to rapidly screen nanoparticles for their transdermal penetration ability. Specifically, this model is built through layer-by-layer alternate printing of blank collagen hydrogel and fibroblasts. Through controlling valve on-time, the spacing between printing lines could be accurately tuned, which could enable modulation of cell infiltration in the future. To confirm the effectiveness of this platform, a 3D construct with one layer of fibroblasts sandwiched between two layers of collagen hydrogel is used to screen silica nanoparticles with different surface charges for their penetration ability, with positively charged nanoparticles demonstrating deeper penetration, consistent with the observation from an existing study involving living skin tissue.