Medium-chain triglyceride reinforce the hepatic damage caused by fructose intake in mice. (January 2019)

• Record Type: Journal Article
• Authors: Guimarães, Janaina; Bargut, Thereza Cristina Lonzetti; Mandarim-de-Lacerda, Carlos Alberto; Aguila, Marcia Barbosa
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 140(2019)
• Page Start: 64
• Page End: 71
• Publication Date: 2019-01
• Subjects: Medium-chain triglycerides -- Fructose -- Steatosis; ACC acetyl-CoA carboxylase -- AIN 93M American Institute of Nutrition's recommendations for rodents to maintain -- ANOVA analysis of variance -- BP blood pressure -- C control group -- CPT carnitine palmitoyltransferase -- C-MCT control with MCT oil group -- F fructose group -- FAS fatty acid synthase -- F-MCT fructose with MCT oil group -- IL interleukin -- MCAD medium-chain acyl-CoA dehydrogenase -- MCFA medium-chain fatty acids -- MCT medium-chain triglycerides -- NAFLD nonalcoholic fatty liver disease -- NASH nonalcoholic steatohepatitis -- NRF nuclear respiratory factor -- OGTT oral glucose tolerance test -- PGC peroxisome proliferator-activated receptor gamma coactivator -- PPAR peroxisome-proliferator-activator receptor -- SREBP sterol regulatory element binding protein -- TC total cholesterol -- TG triglycerides -- TNF tumor necrosis factor -- UCP mitochondrial uncoupling protein; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2018.11.005
• Languages: English
• ISSNs: 0952-3278

Abstract

Highlights: Fructose increased liver lipogenesis and inflammation, and reduced beta-oxidation. MCT and fructose led to marked fat accumulation (steatosis) within the hepatocytes. MCT intake did not revert or attenuate the hepatic damage caused by fructose. Abstract: We aimed to investigate the effects of medium-chain triglyceride oil on the high fructose diet-provoked hepatic abnormalities in mice. We used C57bl/6 mice of 3-months-old divided into four groups for 12 weeks: control (C), control with MCT (C-MCT), fructose (F), and fructose with MCT (F-MCT). We investigated food and water intake, body mass, blood pressure, glucose tolerance, plasma and liver biochemistry, hepatic protein and gene expression. There were no changes in body mass, food intake and glucose tolerance among the groups. The F group presented increased water intake and blood pressure associated with hepatic steatosis and elevated de novo lipogenesis, beta-oxidation, mitochondrial biogenesis and inflammation in the liver. Surprisingly, the C-MCT group also showed hepatic steatosis and inflammation in the liver, and the F-MCT group had no exacerbations of fructose-induced abnormalities, showing marked hepatic steatosis, lipogenesis de novo and hepatic inflammation. The MCT oil groups also presented increased beta-oxidation and mitochondrial biogenesis. In conclusion, MCT oil showed detrimental hepatic effects and should be used with caution, especially in the presence of hepatic alterations.