CYPOR is a novel and independent prognostic biomarker of recurrence‐free survival in triple‐negative breast cancer patients. Issue 3 (7th November 2018)

• Record Type: Journal Article
• Authors: Pedersen, Martin H.; Hood, Brian L.; Ehmsen, Sidse; Beck, Hans C.; Conrads, Thomas P.; Bak, Martin; Ditzel, Henrik J.; Leth‐Larsen, Rikke
• Is Part Of: International journal of cancer. Volume 144:Issue 3(2019)
• Page Start: 631
• Page End: 640
• Publication Date: 2018-11-07
• Subjects: triple‐negative breast cancer -- CYPOR -- mass spectrometry -- formalin‐fixed paraffin‐embedded -- biomarker; Cancer -- Periodicals; Cancer -- Prevention -- Periodicals; 616.994
• DOI: 10.1002/ijc.31798
• Languages: English
• ISSNs: 0020-7136

Abstract

Abstract : Prognostic and predictive biomarkers of disease and treatment outcome are needed to ensure optimal treatment of patients with triple‐negative breast cancer (TNBC). In a mass spectrometry‐based global proteomic study of 44 formalin‐fixed, paraffin‐embedded (FFPE) primary TNBC tumors and 10 corresponding metastases, we found that Cytochrome P450 reductase (CYPOR) expression correlated with patient outcome. The correlation between CYPOR expression and outcome was further evaluated in a Danish cohort of 113 TNBC patients using immunohistochemistry and publicly available gene expression data from two cohorts of TNBC and basal‐like breast cancer patients, respectively ( N = 249 and N = 580). A significant correlation between high CYPOR gene expression and shorter recurrence‐free survival (RFS), but not overall survival, was found in the cohort of 249 TNBC patients ( p = 0.018, HR = 1.77, 95% CI 1.1–2.85), and this correlation was recapitulated in a cohort of 580 basal‐like breast cancer patients ( p = 0.018, HR = 1.4, 95% CI 1.06–1.86). High CYPOR protein expression was also associated with shorter RFS in the cohort of 113 TNBC patients ( p = 0.017, HR = 2.73, 95% CI 1.20–6.19), particularly those who were lymph node tumor‐negative ( p = 0.029, HR = 5.22). Multivariate Cox regression analysis identified CYPOR as an independent prognostic factor for shorter RFS in TNBC patients ( p = 0.032, HR = 2.19, 95% CI 1.07–4.47). Together, these data suggest high expression of CYPOR as an independent prognostic biomarker of shorter RFS, which could be used to identify patients who should receive more extensive adjuvant treatment and more aggressive surveillance. Abstract : What's new? Triple‐negative breast cancer is highly heterogeneous and frequently aggressive, and finding molecular markers that correlate with outcomes can help refine treatment strategies. Previous work has pointed to cytochrome P450 reductase (CYPOR) as a marker associated with patient outcomes for triple‐negative breast cancer. These authors looked at the correlation between CYPOR expression and time to cancer recurrence. Patients with high levels of cytochrome P450 reductase saw their cancers return faster. CYPOR, then, may help identify patients that could benefit from more aggressive treatment and careful monitoring.