Raman characterizations of red blood cells with β-thalassemia using laser tweezers Raman spectroscopy. Issue 39 (September 2018)

• Record Type: Journal Article
• Authors: Jia, Wenguang; Chen, Ping; Chen, Wenqiang; Li, Yongqing
• Other Names: Eskazan. Ahmet Emre section editor.
• Is Part Of: Medicine. Volume 97:Issue 39(2018)
• Publication Date: 2018-09
• Subjects: β-thalassemia -- hemoglobin -- laser tweezers Raman spectroscopy -- Raman spectra -- red blood cell; Medicine -- Periodicals; Medicine -- Periodicals; Médecine -- Périodiques; Geneeskunde; Medicine; Periodicals; Periodicals; 610.5
• DOI: 10.1097/MD.0000000000012611
• Languages: English
• ISSNs: 0025-7974

Abstract

Abstract : Abstract: This study aimed to study the differences in Raman spectra of red blood cells (RBCs) among patients with β-thalassemia and controls using laser tweezers Raman spectroscopy (LTRS) system. A total of 33 patients with β-thalassemia major, 49 with β-thalassemia minor, and 65 controls were studied. Raman spectra of RBCs for each sample were recorded. Principal component analysis (PCA), one-way analysis of variance (ANOVA), and independent-sample t test were performed. The intensities of Raman spectra of β-thalassemia (major and minor) RBCs were lower than those of controls, especially at bands 1546, 1603, and 1619 cm –1 . The intensity ratio of band 1546 cm –1 to band 1448 cm –1 demonstrated that there was a significant difference between the spectra of β-thalassemia major (mostly below 2.15) and those of controls. The spectra of controls could be well distinguished from those of β-thalassemia major using PCA. After normalization, the spectra of two different genotypes with β 0 /β 0 mutations mainly overlapped, while those with β + /β + mutations had lower intensity at bands 1546, 1603, and 1619 cm –1 . The present study provided Raman characteristics of RBCs in patients with β-thalassemia major and supported the use of LTRS as a method for screening β-thalassemia major. The recognition rate for β-thalassemia minor needs to be further improved.