The determination of polycyclic aromatic hydrocarbons in human urine by high‐resolution gas chromatography–mass spectrometry. (16th January 2018)

• Record Type: Journal Article
• Authors: Cho, Sung‐Hee; Lee, Sun‐Kyung; Kim, Chong Hyeak
• Is Part Of: Biomedical chromatography. Volume 32:Number 5(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-01-16
• Subjects: carcinogenesis -- gas chromatography -- high‐resolution mass spectrometry -- polycyclic aromatic hydrocarbons -- urine; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.4166
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Polycyclic aromatic hydrocarbons (PAHs), organic compounds formed by at least two condensed aromatic rings, are ubiquitous environmental pollutants that are produced by incomplete combustion of organic materials. PAHs have been classified as carcinogenIC to humans by the International Agency for Research on Cancer, because they can bind to DNA, causing mutations. Therefore, the levels of PAHs in human urine can be used as an indicator for potential carcinogenesis and cell mutation. An analytical method was developed for the accurate measurement of PAHs in urine using high‐resolution gas chromatography–mass spectrometry. Urine samples were extracted by an Oasis HLB extraction cartridge after enzymatic hydrolysis with a β ‐glucuronidase/arylsulfatase cocktail. The 18 PAHs were separated using an Agilent DB‐5 MS capillary column (30 m × 0.25 mm, 0.25 μm) and monitored by time‐of‐flight mass spectrometry. Under the optimized method, the linearity of calibration curves was >0.994. The limits of detection at a signal‐to‐noise ratio of 3 were 10–100 ng/L. The coefficients of variation were in the range of 0.4–9.0%. The present method was highly accurate for simultaneous determination of 18 PAHs in human urine and could be applied to monitoring and biomedical investigations to check exposure of PAHs.