Carbohydrate-derived fulvic acid is a highly promising topical agent to enhance healing of wounds infected with drug-resistant pathogens. Issue 4 (October 2015)

• Record Type: Journal Article
• Authors: Zhao, Yanan; Paderu, Padmaja; Delmas, Guillaume; Dolgov, Enriko; Lee, Min Hee; Senter, Meredith; Park, Steven; Leivers, Stephen; Perlin, David S.
• Is Part Of: Journal of trauma and acute care surgery. Volume 79:Issue 4(2015:Jun.)Supplement 2
• Publication Date: 2015-10
• Subjects: CHD-FA -- wound infections -- cutaneous wound -- rat model; Surgical intensive care -- Periodicals; Surgical emergencies -- Periodicals; Wounds and injuries -- Surgery -- Periodicals; 617.026
• DOI: 10.1097/TA.0000000000000737
• Languages: English
• ISSNs: 2163-0755

Abstract

Abstract : BACKGROUND: This work was intended as a proof-of-principle study to help establish carbohydrate-derived fulvic acid (CHD-FA) as a safe and effective agent that can be deployed to prevent the onset of drug-resistant bacterial and fungal infections in military and civilian personnel experiencing traumatic wound. METHODS: Minimum inhibitory concentrations for CHD-FA were established on a total of 500 clinical isolates representing wound-associated drug-sensitive and drug-resistant bacterial and fungal pathogens. The efficacy of early use of CHD-FA to enhance healing of wounds infected with methicillin-resistant Staphylococcus aureus or Pseudomonas aeruginosa was evaluated in an in vivo rat model. RESULTS: CHD-FA showed strong activity against a variety of bacterial and fungal pathogens with minimum inhibitory concentration values equal or less than 0.5%. Compared with infected but untreated wounds, improved wound healing upon CHD-FA treatment was observed in both infection models, demonstrated by wound surface area measurement, histopathologic examination, and expression profiling of wound healing genes. Up-regulation of proinflammatory cytokine interleukin 6 (IL-6) at Day 3 after infection was significantly dampened at Days 6 and 10 in the CHD-FA–treated wounds in both infection models, displaying an improved and accelerated wound healing. CONCLUSION: CHD-FA is a promising topical remedy for drug-resistant wound infections. It accelerated the healing process of wounds infected with methicillin-resistant S. aureus and multidrug-resistant P. aeruginosa in rats, which is linked to both its antimicrobial and anti-inflammatory properties.