A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells. Issue 21 (1st November 2015)

• Record Type: Journal Article
• Authors: Chandran, Yogeswari; Kang, Nam-Young; Park, Sung-Jin; Husen Alamudi, Samira; Kim, Jun-Young; Sahu, Srikanta; Su, Dongdong; Lee, Jungyeol; Vendrell, Marc; Chang, Young-Tae
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 25:Issue 21(2015)
• Page Start: 4862
• Page End: 4865
• Publication Date: 2015-11-01
• Subjects: Mouse embryonic stem cells -- Fluorescence imaging -- Single cell PCR -- Fluorescence-assisted cell sorting -- Three germ layers; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2015.06.037
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9 ) with high selectivity for mouse embryonic stem cells.CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.