Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC. (10th December 2015)

• Record Type: Journal Article
• Authors: Wu, Ding-Tao; Cheong, Kit-Leong; Deng, Yong; Lin, Peng-Cheng; Wei, Feng; Lv, Xiao-Jie; Long, Ze-Rong; Zhao, Jing; Ma, Shuang-Cheng; Li, Shao-Ping
• Is Part Of: Carbohydrate polymers. Volume 134(2015)
• Page Start: 12
• Page End: 19
• Publication Date: 2015-12-10
• Subjects: Lycium barbarum -- Wolfberry -- Polysaccharide -- Saccharide mapping -- PACE -- HPTLC; ANTS 8-aminonaphthalene-1, 3, 6-trisulfonic acid -- HPTLC high-performance thin-layer chromatography -- PACE polysaccharide analysis by using carbohydrate gel electrophoresis -- SMC simulative mean chromatogram -- TFA trifluoroacetic acid; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.07.052
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Polysaccharides from L. barbarum in China were compared using saccharide mapping. Composite enzymatic digestion followed by PACE and HPTLC analysis was performed. High similarity of polysaccharides from L. barbarum in China was found. Abstract: Water-soluble polysaccharides from 51 batches of fruits of L. barbarum (wolfberry) in China were investigated and compared using saccharide mapping, partial acid hydrolysis, single and composite enzymatic digestion, followed by polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) analysis and high performance thin layer chromatography (HPTLC) analysis, respectively. Results showed that multiple PACE and HPTLC fingerprints of partial acid and enzymatic hydrolysates of polysaccharides from L. barbarum in China were similar, respectively. In addition, results indicated that β-1, 3-glucosidic, α-1, 4-galactosiduronic and α-1, 5-arabinosidic linkages existed in polysaccharides from L. barbarum collected in China, and the similarity of polysaccharides in L. barbarum collected from different regions of China was pretty high, which are helpful for the improvement of the performance of polysaccharides from L. barbarum in functional/health foods area. Furthermore, polysaccharides from Panax notoginseng, Angelica sinensis, and Astragalus membranaceus var. mongholicus were successfully distinguished from those of L. barbarum based on their PACE fingerprints. These results were beneficial to improve the quality control of polysaccharides from L. barabrum and their products, which suggested that saccharide mapping based on PACE and HPTLC analysis could be a routine approach for quality control of polysaccharides.