Immunomodulatory activity on macrophage of a purified polysaccharide extracted from Laminaria japonica. (10th December 2015)

• Record Type: Journal Article
• Authors: Fang, Qing; Wang, Jing-Fei; Zha, Xue-Qiang; Cui, Shao-Hua; Cao, Lei; Luo, Jian-Ping
• Is Part Of: Carbohydrate polymers. Volume 134(2015)
• Page Start: 66
• Page End: 73
• Publication Date: 2015-12-10
• Subjects: Laminaria japonica -- Polysaccharides -- Immunostimulation -- Macrophage -- TLR4; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.07.070
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A homogenous polysaccharide (LJP-31) was isolated from Laminaria japonica . The basic structure features of LJP-31 were characterized. The activation of macrophages by LJP-31 and the related mechanism were studied. Abstract: In this work, a novel water-soluble homogeneous polysaccharide (LJP-31) with a molecular mass of 2.24 × 10 6 Da was isolated and purified from Laminaria japonica using DEAE-cellulose and Sephacryl S500 chromatography. Results showed that LJP-31 mainly consists of arabinose, mannose, glucose and galactose in a molar ratio of 1.0:7.8:6.6:0.8. LJP-31 exhibited significant stimulation on macrophages and enhanced the production of NO, TNF-α, IL-1β, IL-6 and IL-10 as well as the up-regulation of their gene expressions. Western blot analysis suggested that LJP-31 has the positive effects on the translocation of NF-κB p65 from cytoplasm to nucleus and the phosphorylation of IκBα, ERK1/2, JNK1/2 and P38 in macrophages. Flow cytometric and confocal laser-scanning microscopy analysis indicated that toll-like receptor 4 (TLR4) was at least one of the recognition receptors of LJP-31 on the plasma membrane of macrophages. Taken together, LJP-31 may exert its immunostimulating potency via TLR4 activation of MAPK and NF-κB signaling pathways.