Single‐shot speckle correlation fluorescence microscopy in thick scattering tissue with image reconstruction priors. Issue 3 (30th January 2018)

• Record Type: Journal Article
• Authors: Chang, Julie; Wetzstein, Gordon
• Is Part Of: Journal of biophotonics. Volume 11:Issue 3(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-01-30
• Subjects: computational imaging -- deep tissue imaging -- fluorescence microscopy -- phase retrieval -- speckle correlation; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201700224
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Deep tissue imaging in the multiple scattering regime remains at the frontier of fluorescence microscopy. Speckle correlation imaging (SCI) can computationally uncover objects hidden behind a scattering layer, but has only been demonstrated with scattered laser illumination and in geometries where the scatterer is in the far field of the target object. Here, SCI is extended to imaging a planar fluorescent signal at the back surface of a 500‐μm‐thick slice of mouse brain. The object is reconstructed from a single snapshot through phase retrieval using a proximal algorithm that easily incorporates image priors. Simulations and experiments demonstrate improved image recovery with this approach compared to the conventional SCI algorithm. Abstract : Speckle correlation imaging exploits properties of random speckle patterns to computationally uncover objects hidden behind a scattering layer from a single snapshot. Here, this technique is extended to image a planar fluorescent object through a thick layer of scattering tissue. The image reconstruction process is reformulated with a proximal algorithm incorporating image priors that yields improved image recovery compared to the previously used algorithm.