Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure. (October 2015)

• Record Type: Journal Article
• Authors: Lin, Chunmei; Yon, Jung-Min; Lee, Beom Jun; Kang, Jong-Koo; Yun, Young Won; Nam, Sang-Yoon
• Is Part Of: Journal of functional foods. Volume 18:Part A(2016)
• Page Start: 617
• Page End: 630
• Publication Date: 2015-10
• Subjects: Punicalagin -- Nicotine -- Teratogenesis -- Placenta -- Yolk sac -- Vascularization; ROS reactive oxygen species -- TBARS thiobarbituric acid reactive substances -- MDA malondialdehyde -- SOD1 cytoplasmic superoxide dismutase -- GPx1 cytoplasmic glutathione peroxidase -- Bcl-xL B-cell lymphoma-extra large -- Bax Bcl2-associated X protein -- TGF-β1 transforming growth factor-beta 1 -- HIF-1α hypoxia inducible factor-1α -- TNF-α tumour necrosis factor-alpha -- IL-1β interleukin 1 beta -- NF-κB nuclear factor kappa-light-chain-enhancer of activated B cells -- IGF-1 insulin-like growth factor-1 -- VEGFα vascular endothelial growth factor alpha -- GAPDH glyceraldehyde-3-phosphate dehydrogenase; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.08.029
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Nicotine (N) induced severe foetal anomalies and placenta-yolk sac circulation defects in mice. N altered regulations of hypoxia- and vasculogenesis-related genes in placenta-yolk sac. N altered regulations of oxidative stress, apoptosis, and inflammation in embryos and placentas. However, punicalagin improved remarkably all these changes induced by N. Punicalagin might act as a preventive agent against foetal anomalies and placental defects by N. Abstract: Punicalagin is a functional ingredient in pomegranate juice, which has various beneficial effects for health and prevention of disease. In this study, the anti-teratogenic effect of punicalagin (1 × 10 −5 or 1 × 10 −4 µM) was investigated in cultured mouse embryos and yolk sac-placentas exposed to nicotine (1 mM), one of the main toxins in cigarette smoke. Nicotine-treated embryos revealed severe anomalies as well as impaired yolk sac vascularization, reduced labyrinth formation, and developmental arrest of blood islands in the chorioallantoic border. Furthermore, nicotine significantly altered the regulations of hypoxia- and vascularization-related genes in yolk sac-placenta and the levels of oxidative stress, apoptosis, and inflammation in both embryos and yolk sac-placentas. However, these detrimental changes were remarkably improved in response to co-treatment with punicalagins. These findings indicate that punicalagin could be crucial to the development of clinical strategies to attenuate nicotine-induced teratogenesis in embryos.