Cellulose alters the expression of nuclear factor kappa B-related genes and Toll-like receptor-related genes in human peripheral blood mononuclear cells. (October 2015)

• Record Type: Journal Article
• Authors: Vogt, Leonie M.; Boekschoten, Mark V.; de Groot, Philip J.; Faas, Marijke M.; de Vos, Paul
• Is Part Of: Journal of functional foods. Volume 18:Part A(2016)
• Page Start: 520
• Page End: 531
• Publication Date: 2015-10
• Subjects: ATM ataxia telangiectasia mutated -- AUC area under the curve -- CD cluster of differentiation -- ECIS electric cell substrate impedance sensing -- EU endotoxin units -- FBS foetal bovine serum -- HEK human embryonic kidney -- IL-1Ra interleukin 1 receptor antagonist -- IRAK1 and interleukin-1 receptor-associated kinase 1 -- JNK c-Jun N-terminal kinases -- LBP lipopolysaccharide-binding protein -- LTA lipoteichoic acid -- MAP2K3 mitogen-activated protein kinase kinase 3 -- MAPK13 mitogen-activated protein kinase 13 -- MyD88 Myeloid Differentiation Factor 88 -- NF-κB nuclear factor kappa B -- NK natural killer -- PIK3cG phosphatidylinositol-4, 5-bisphosphate 3-kinase -- PIK3R1 phosphoinositide-3-kinase regulatory subunit 1 -- PBMC peripheral blood mononuclear cell -- PMA phorbol 12-myristate 13-acetate -- PRR pattern recognition receptor -- SEAP secreted embryonic alkaline phosphatase -- TEER trans epithelial electrical resistance -- TLR Toll-like receptor -- TNF tumor necrosis factor -- TRIF TIR-domain-containing adapter-inducing interferon-β -- VST Variance Stabilisation Transformation; Cellulose -- Dietary fibre -- NF-κB -- TLR; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.08.011
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Stimulation with cellulose activates NF-κB in MyD88/TLR-dependent and independent ways. Cellulose induces activation of TLR2 and TLR4. Gene expression in human peripheral leukocytes is altered upon stimulation with cellulose. Cellulose could thus be a valuable component of root vegetable byproduct. Abstract: The immunomodulatory and epithelial barrier effects of cellulose as a dietary fibre were studied to analyse the potential for use in health promoting functional foods. Reporter assays demonstrated cellulose-mediated activation through TLR/MyD88 dependent-, and independent pathways. Microchip analysis of human PBMCs showed that cellulose induced upregulation of three NF-κB related genes, i.e. CD40 molecule, interleukin 1 receptor antagonist (IL-1Ra), and interleukin-1 receptor-associated kinase 1 (IRAK1). Five upregulated genes related specifically to TLR signalling were identified, i.e. interleukin 1 receptor antagonist (IL-1Ra), interleukin-1 receptor-associated kinase 1 (IRAK1), jun proto-oncogene, mitogen-activated protein kinase kinase 3 (MAP2K3), and mitogen-activated protein kinase 13 (MAPK13). Cellulose did not affect T84 intestinal epithelial cell resistance. Cellulose does not directly affect T84 cell barrier function. However, it alters gene expression in human immune cells and activates TLR and non-TLR related pattern recognition pathways, indicating the immunomodulatory potential of cellulose as major component of root pulp byproduct.