Prowashonupana barley dietary fibre reduces body fat and increases insulin sensitivity in Caenorhabditis elegans model. (October 2015)

• Record Type: Journal Article
• Authors: Gao, Chenfei; King, Michael L.; Fitzpatrick, Zachary L.; Wei, Wenqian; King, Jason F.; Wang, Mingming; Greenway, Frank L.; Finley, John W.; Burton, Jeffrey H.; Johnson, William D.; Keenan, Michael J.; Enright, Frederick M.; Martin, Roy J.; Zheng, Jolene
• Is Part Of: Journal of functional foods. Volume 18:Part A(2016)
• Page Start: 564
• Page End: 574
• Publication Date: 2015-10
• Subjects: IFD intestinal fat deposition -- PWB Prowashonupana barley -- PPR pharyngeal pumping rate; Obesity -- Barley -- Animal models -- Insulin sensitivity -- Ageing; Beta glucan (PubChem CID: 46173706) -- Glucose (PubChem CID: 79025) -- Vitamin B1 (Thiamin) (PubChem CID: 1130) -- Vitamin B3 (Niacin) (PubChem CID: 938); Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.08.014
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Prowashonupana barley (PWB) is rich in β-glucan and dietary fermentable fibre. PWB consumption reduced intestinal fat deposition in the C. elegans model organism. PWB consumption improved healthspan in C. elegans . PWB may help to reduce the worldwide obesity epidemic and the cost of health care. Abstract: Prowashonupana barley (PWB) is high in β-glucan with moderate content of resistant starch. PWB reduced intestinal fat deposition (IFD) in wild type Caenorhabditis elegans ( C. elegans, N2), and in sir-2.1 or daf-16 null mutants, and sustained a surrogate marker of lifespan, pharyngeal pumping rate (PPR), in N2, sir-2.1, daf-16, or daf-16/daf-2 mutants. Hyperglycaemia (2% glucose) reversed or reduced the PWB effect on IFD in N2 or daf-16/daf-2 mutants with a sustained PPR. mRNA expression of cpt-1, cpt-2, ckr-1, and gcy-8 were dose-dependently reduced in N2 or daf-16 mutants, elevated in daf-16/daf-2 mutants with reduction in cpt-1, and unchanged in sir-2.1 mutants. mRNA expressions were increased by hyperglycaemia in N2 or daf-16/daf-2 mutants, while reduced in sir-2.1 or daf-16 mutants. The effects of PWB in the C. elegans model appeared to be primarily mediated via sir-2.1, daf-16, and daf-16 / daf-2 . These data suggest that PWB and β-glucans may benefit hyperglycaemia-impaired lipid metabolism.