Anti-allergic effect of α-cubebenoate isolated from Schisandra chinensis using in vivo and in vitro experiments. (15th September 2015)

• Record Type: Journal Article
• Authors: Lee, Kyoung-Pil; Kang, Saeromi; Park, Soo-Jin; Kim, Jung-Min; Lee, Jung-Min; Lee, Ae-Yeon; Chung, Hae-Young; Choi, Young-Whan; Lee, Young-Geun; Im, Dong-Soon
• Is Part Of: Journal of ethnopharmacology. Volume 173(2015)
• Page Start: 361
• Page End: 369
• Publication Date: 2015-09-15
• Subjects: OVA ovalbumin -- IL-4 interleukin-4 -- IL-13 interleukin-13 -- TGF-β1 transforming growth factor beta-1 -- BALF bronchoalveolar fluid -- PAS periodic acid-schif -- H&E hematoxylin and eosin -- [Ca2+]i intracellular Ca2+ concentration -- ELISA enzyme-linked immunosorbent assay -- DNP dinitrophenyl -- DNP-hAb human dinitrophenyl albumin -- PBS phosphate-buffered saline -- PCR polymerase-chain reaction -- HRP horseradish peroxidase -- GAPDH glyceraldehyde-3-phosphate dehydrogenase; α-cubebenoate -- Anti-allergy -- Asthma -- Mast cells -- Schisandra chinensis; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2015.07.049
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Aim of the study: In Oriental countries, the dried fruits of Schisandra chinensis are extensively used in traditional medicine to treat asthma, gonorrhea, and other diseases. Recently, α-cubebenoate was isolated as an anti-inflammatory component from Schisandra chinensis . In the present study, the authors examined the anti-allergic effect of α-cubebenoate using in vivo and in vitro experiments. Materials and methods: α-Cubebenoate was isolated from an extract of Schisandra chinensis fruits. Antigen-induced degranulation and Ca 2+ mobilization were measured in RBL-2H3 mast cells. In addition, BALB/c mice were sensitized with ovalbumin and aluminum hydroxide, and then challenged with ovalbumin for three consecutive days. α-Cubebenoate (1 mg/kg) was administered intraperitoneally 30 min before each ovalbumin challenge. Results: In RBL-2H3 mast cells, α-cubebenoate inhibited antigen-induced degranulation and increase of intracellular Ca 2+ concentrations. In the ovalbumin-induced asthma model, α-cubebenoate suppressed bronchiolar structural changes induced by ovalbumin challenge. Furthermore, α-cubebenoate strongly inhibited accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid. α-Cubebenoate also suppressed Th2 cytokines (IL-4 and IL-13) and TGF-β1 in lung tissues and in immune cells at the mRNA and protein levels. Conclusion: α-Cubebenoate has an inhibitory effect on allergic inflammation and could be utilized as an agent for the treatment of asthma. Graphical abstract: