Anti-obesity and anti-hepatosteatosis effects of dietary scopoletin in high-fat diet fed mice. (August 2016)

• Record Type: Journal Article
• Authors: Ham, Ju Ri; Lee, Hae-In; Choi, Ra-Yeong; Sim, Mi-Ok; Choi, Myung-Sook; Kwon, Eun-Young; Yun, Kyeong Won; Kim, Myung-Joo; Lee, Mi-Kyung
• Is Part Of: Journal of functional foods. Volume 25(2016)
• Page Start: 433
• Page End: 446
• Publication Date: 2016-08
• Subjects: High-fat -- Non-alcoholic liver disease -- Obesity -- Scopoletin -- Transcriptome; ALT alanine aminotransferase -- Apoa4 apolipoprotein A-IV -- AST aspartate aminotransferase -- β-oxidation fatty acid β-oxidation -- Cdkn1a cyclin-dependent kinase inhibitor 1A -- Cidea cell death-inducing DFFA-like effector A -- Col1a1 collagen, type 1, alpha 1 -- CPT carnitine palmitoyltransferase -- Cyp7a1 cholesterol 7 alpha-hydroxylase -- Errfi1 ERBB receptor feedback inhibitor 1 -- FAS fatty acid synthase -- FFA free fatty acid -- G6P glucose-6-phosphate -- G6Pase glucose-6-phosphatase -- Gapdh glyceraldehyde-3-phosphate dehydrogenase -- Gdf15 growth differentiation factor 15 -- GK glucokinase -- HDL-C HDL-cholesterol -- H&E haematoxylin and eosin -- HF high-fat diet -- HF-LS high-fat diet with 0.01% scopoletin -- HF-HS high-fat diet with 0.05% scopoletin -- HOMA-IR homeostatic index of insulin resistance -- IL-1β interleukin-1 beta -- IL-6 interleukin-6 -- IFNγ interferon gamma -- IPA ingenuity pathway analysis -- IPGTT intraperitoneal glucose tolerance test -- IR insulin resistance -- MCP-1 monocyte chemoattractant protein-1 -- Mmp13 matrix metallopeptidase 13 -- NAD+ nicotinamide adenine dinucleotide+ -- NADH nicotinamide adenine dinucleotide -- NADPH nicotinamide adenine dinucleotide phosphate -- NC normal diet -- NAFLD non-alcoholic fatty liver disease -- PAP phosphatidate phosphohydrolase -- PEPCK phosphoenolpyruvate carboxykinase -- Saa1 serum amyloid A1 -- TC total cholesterol -- TG triacylglycerol -- Trichrome Masson's trichrome -- TNFα tumour necrosis factor alpha; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.06.026
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Scopoletin attenuates non-alcoholic fatty liver disease in high-fat diet fed mice. Scopoletin identified 3 gene networks related to lipid metabolism and inflammation. Target genes of lipid metabolism are Cidea, Apoa4, Cyp7a1 and Errfi1 . Target genes of inflammation are Col1a1, Mmp13, Cdkn1a and Saa1. Abstract: The effects of scopoletin on non-alcoholic fatty liver in obese mice were investigated. Mice were fed high-fat diet (HF) with or without two doses of scopoletin (0.01 and 0.05%, w/w) for 16 weeks. Both doses of scopoletin led to similar reductions in body weight, visceral fat, serum levels of leptin, lipid, TNFα, IL-6, IFNγ and MCP-1, insulin resistance and hepatic lipid accumulation, whereas they increased serum adiponectin and faecal lipid levels. Ingenuity pathway analysis revealed that hepatic gene networks related to lipid concentrations, inflammation of organs, quantity of adipose tissue, proliferation of cell and necrosis were down-regulated in the scopoletin group. The top up- or down-regulated genes were Cidea, Apoa4, Cyp7a1, Errfi1, Col1a1, Mmp13, Cdkn1a, Gdf15 and Saa1, which emerged as associated genes related to hepatic steatosis and inflammation. These results indicate that scopoletin may ameliorate HF-induced hepatic dysfunction via regulation of lipid metabolic and inflammatory genes.