Novel pH-responsive and self-assembled nanoparticles based on Bletilla striata polysaccharide: preparation and characterization. Issue 70 (3rd December 2018)

• Record Type: Journal Article
• Authors: Zhu, Junxiao; Guo, Xiaoxi; Guo, Tingting; Yang, Ye; Cui, Xiuming; Pan, Jun; Qu, Yuan; Wang, Chengxiao
• Is Part Of: RSC advances. Volume 8:Issue 70(2018)
• Page Start: 40308
• Page End: 40320
• Publication Date: 2018-12-03
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra07202g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : In this investigation, innovative pH-sensitive and amphiphilic nanoparticles (NPs) were synthesized by grafting histidine (His, pH sensitive molecule) and stearic acid (SA, hydrophobic segment) onto the polysaccharides of Bletilla striata (BSP). Abstract : In this investigation, innovative pH-sensitive and amphiphilic nanoparticles (NPs) were synthesized by grafting histidine (His, pH sensitive molecule) and stearic acid (SA, hydrophobic segment) onto the polysaccharides of Bletilla striata (BSP). The His-SA-BSP was able to self-assemble into NPs with pH sensitivity. The acidic conditions could trigger the imidazole ionization and reverse the surface charge, while the electrostatic repulsion wrecked the structure and drove the NPs to a swollen state, as revealed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and critical micelle concentration (CMC) analyses. By increasing the degree of substitution (DS) of His, the NPs showed improved pH sensitivity. The NPs could accelerate Doxorubicin (Dox) release to a remarkably greater extent (3-fold) at pH 5 than at pH 7.4. The CCK-8 assay demonstrated a good biocompatibility of the NPs towards different cell lines and a specific inhibition effect of Dox-loaded NPs against tumor cells. Furthermore, the NPs showed the improved cellular uptake of Dox towards MCF-7 by fluorescence microscopy and flow cytometry. Therefore, the new His-SA-BSP showed potential applications in drug nanocarrier systems.